The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

Lutz Christian Gerhardt; Kate L. Widdows; Melek M. Erol; Charles W. Burch; José A. Sanz-Herrera; Ignacio Ochoa; Rolf Stämpfli; Iman S. Roqan; Simon Gabe; Tahera Ansari; Aldo R. Boccaccini

doi:10.1016/j.biomaterials.2011.02.032

The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

Lutz Christian Gerhardt, Kate L. Widdows, Melek M. Erol, Charles W. Burch, José A. Sanz-Herrera, Ignacio Ochoa, Rolf Stämpfli, Iman S. Roqan, Simon Gabe, Tahera Ansari, Aldo R. Boccaccini^*

^*Bu çalışma için yazışmadan sorumlu yazar

Kimya Mühendisliği Bölümü

Araştırma sonucu: ???type-name??? › Makale › bilirkişi

181 Atıf (Scopus)

Özet

The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10^-9 m²) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct.

Orijinal dil	İngilizce
Sayfa (başlangıç-bitiş)	4096-4108
Sayfa sayısı	13
Dergi	Biomaterials
Hacim	32
Basın numarası	17
DOI'lar	https://doi.org/10.1016/j.biomaterials.2011.02.032
Yayın durumu	Yayınlandı - Haz 2011

Finansman

The authors acknowledge the financial support of King Abdullah University of Science and Technology (KAUST). Special thanks are due to Dr. L. Serra (Roche, Germany), Dr. J. Lackey (Insight Biotechnology, UK), C. Akufo-Addo (LGC Standards, UK), Dr. M. Ardakani, E. Ware, R. Chater (all Imperial College London) for technical support and helpful discussions. The authors also acknowledge Prof. W. Stark and D. Mohn (ETH Zurich) for providing the nano-sized BG used.

Finansörler	Finansör numarası
King Abdullah University of Science and Technology

Belgeye Erişim

10.1016/j.biomaterials.2011.02.032

Diğer Dosyalar ve Linkler

Link to publication in Scopus

Alıntı Yap

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title = "The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds",

abstract = "The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct.",

keywords = "Angiogenesis, Bioactive glass, Bone, Composite scaffold, Vascular endothelial growth factor, Vascularisation",

author = "Gerhardt, {Lutz Christian} and Widdows, {Kate L.} and Erol, {Melek M.} and Burch, {Charles W.} and Sanz-Herrera, {Jos{\'e} A.} and Ignacio Ochoa and Rolf St{\"a}mpfli and Roqan, {Iman S.} and Simon Gabe and Tahera Ansari and Boccaccini, {Aldo R.}",

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doi = "10.1016/j.biomaterials.2011.02.032",

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T1 - The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

AU - Gerhardt, Lutz Christian

AU - Widdows, Kate L.

AU - Erol, Melek M.

AU - Burch, Charles W.

AU - Sanz-Herrera, José A.

AU - Ochoa, Ignacio

AU - Stämpfli, Rolf

AU - Roqan, Iman S.

AU - Gabe, Simon

AU - Ansari, Tahera

AU - Boccaccini, Aldo R.

PY - 2011/6

Y1 - 2011/6

N2 - The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct.

AB - The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct.

KW - Angiogenesis

KW - Bioactive glass

KW - Bone

KW - Composite scaffold

KW - Vascular endothelial growth factor

KW - Vascularisation

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AN - SCOPUS:79953292885

SN - 0142-9612

VL - 32

SP - 4096

EP - 4108

JO - Biomaterials

JF - Biomaterials

IS - 17

ER -

The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

Özet

Finansman

Belgeye Erişim

Diğer Dosyalar ve Linkler

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