The effect of thiolated phospholipids on formation of supported lipid bilayers on gold substrates investigated by surface-sensitive methods

Abdulhalim Kılıç, M. Fazeli Jadidi, Hakan Özgür Özer, Fatma Neşe Kök*

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: ???type-name???Makalebilirkişi

11 Atıf (Scopus)

Özet

Most of the model lipid membrane studies on gold involve the usage of various surface-modification strategies to rupture liposomes and induce lipid bilayer formation since liposomes with polar surfaces do not interact with bare, hydrophobic gold. In this study, a thiol-modified phospholipid, 1,2-Dipalmitoyl-sn-Glycero-3-Phosphothioethanol (DPPTE) was incorporated into phosphatidylcholine (PC) based liposomes to form supported lipid bilayer (SLB) on gold surfaces without further modification. The binding kinetics of liposomes with different DPPTE ratio (0.01 to 100% mol/mol) and diameters were monitored by Quartz Crystal Microbalance with Dissipation (QCM-D). The dissipation change per frequency change, i.e. acoustic ratio, which is evaluated as a degree of the viscoelasticity, considerably decreased with the presence of DPPTE (from 162.3 GHz−1 for flattened PC liposomes to ca. 89.5 GHz−1 for 100% DPPTE liposomes) when compared to the results of two reference rigid monolayers and two viscoelastic layers. To assess the quality of SLB platform, the interpretation of QCM-D data was also complemented with Surface Plasmon Resonance. The optimum thiolated-lipid ratio (1%, lower thiol ratio and higher rigidity) was then used to determine the dry-lipid mass deposition, the water content and the thickness values of the SLB via viscoelastic modelling. Further surface characterization studies were performed by Atomic Force Microscopy with high spatial resolution. The results suggested that model membrane was almost continuous with minimum defects but showed more dissipative/soft nature compared to an ideal bilayer due to partially fused liposomes/overlapped lipid bilayers/multilayer islands. These local elevations distorted the planarity and led the increase of overall membrane thickness to ∼7.0 nm.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)117-125
Sayfa sayısı9
DergiColloids and Surfaces B: Biointerfaces
Hacim160
DOI'lar
Yayın durumuYayınlandı - 1 Ara 2017

Bibliyografik not

Publisher Copyright:
© 2017 Elsevier B.V.

Finansman

Financially support of Istanbul Technical University Scientific Research Projects Unit (ITU-BAP, 36172 ) is gratefully acknowledged.

FinansörlerFinansör numarası
ITU-BAP36172
Firat University Scientific Research Projects Management Unit

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