Synthesis, biocompatibility and gene encapsulation of poly(2-Ethyl 2-Oxazoline)-dioleoyl phosphatidylethanolamine (PEtOx-DOPE) and post-modifications with peptides and fluorescent dye coumarin

Sevgi Gulyuz, Duygu Bayram, Umut Ugur Ozkose, Zeynep Busra Bolat, Polen Kocak, Ongun Mehmet Saka, Burcu Devrim, Melek Parlak Khalily, Dilek Telci, Fikrettin Sahin, Salih Özçubukçu, Esma Sezer, Mehmet Atilla Tasdelen, Onur Alpturk, Asuman Bozkır, Ozgur Yilmaz*

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: ???type-name???Makalebilirkişi

9 Atıf (Scopus)

Özet

Liposome surface modifications serve great potential applications of liposomes, for instance, increasing stability, bioactive liposome conjugates, and targeted drug, gene, and image agent delivery. In this study, novel targeted lipopolymers, peptide 18/peptide 563-poly(2-ethyl-2-oxazoline)-dioleoylphosphatidyl-ethanolamine (P18/P563-PEtOx-DOPE), have been demonstrated to be successfully synthesized. The structures of P18/P563-PEtOx-DOPE were confirmed by FT-IR spectroscopy, GPC, and 1H-NMR. In this strategy, poly(2-ethyl 2-oxazoline)-modified liposomes were firstly constructed with molecular weights of 3,500 and 5,800 Da. Then, we chose PEtOx5800-DOPE because it has been obtained better particle size (88.74 ± 0.6816) according to the DLS results. Then, peptides- and dye-PEtOx lipid-based nanovesicle (LN) were prepared by peptide-18, peptide-563, and 7-mercapto-4-methyl coumarin. Genetic material (pDNA) was encapsulated into the liposomes and evaluated the encapsulation of plasmid DNA with migration by using agarose gel electrophoresis. In vitro cytotoxicity experiment results on prostate cancer and breast cancer cell lines, parallelly with the healthy prostate (PNT1A) and breast (MCF10A) epithelial cell lines, cells showed insignificant toxic effects. Thus, we can suggest a novel PEtOx phospholipid thanks to this article and its integration with ligands, which great potential for gene transfer system.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)1-13
Sayfa sayısı13
DergiInternational Journal of Polymeric Materials and Polymeric Biomaterials
DOI'lar
Yayın durumuYayınlandı - 2020

Bibliyografik not

Publisher Copyright:
© 2020, © 2020 Taylor & Francis Group, LLC.

Finansman

The present work was financially supported by Turkish Scientific and Technological Council [TUBITAK-213M725] and ITU BAP (project number: TDK-2019-41824).

FinansörlerFinansör numarası
ITU BAPTDK-2019-41824
Turkish Scientific and Technological CouncilTUBITAK-213M725

    Parmak izi

    Synthesis, biocompatibility and gene encapsulation of poly(2-Ethyl 2-Oxazoline)-dioleoyl phosphatidylethanolamine (PEtOx-DOPE) and post-modifications with peptides and fluorescent dye coumarin' araştırma başlıklarına git. Birlikte benzersiz bir parmak izi oluştururlar.

    Alıntı Yap