Spectroscopic studies of the interaction between isolated polyphenols from coffee and the milk proteins

Tamer M. El-Messery, Eman A. Mwafy*, Ayman M. Mostafa, Hala M.Fakhr El-Din, Abeer Mwafy, Ryszard Amarowicz, Beraat Ozçelik

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: Dergiye katkıMakalebilirkişi

36 Atıf (Scopus)

Özet

In this work, the binding of polyphenols isolated from coffee (PIC) with milk protein fractions was investigated by using different spectroscopic techniques as Fourier Transform Infrared Spectroscopic technique, high-performance liquid chromatography, and fluorescence quenching emission method. The polyphenols fraction was isolated from coffee powder using a chromatographic column packed with lipophilic Sephadex LH-20 gel, while there are different types of milk proteins skim milk 3% protein (SM), acid casein (β-Casein (β-CN), α-Casein (α-CN), κ-Casein (κ-CN)), and whey protein isolate (WPI) (β-lactoglubulin (β-Lg) and α-lactalbumin (α-La)) were investigated. From our study, the optimum pH for precipitating complex from the interaction of polyphenols with milk proteins, WPI, acid casein was 5, 8, and 7, respectively. Also, the fluorescence quenching method for polyphenols-proteins interaction exhibited fluorescence quenching in the descending order of α-CN > β-Lg > SM3% > WPI > α-La > acid casein > β-CN > κ-CN for milk proteins. Furthermore, vibrational absorption mode of the functional groups confirmed of the appearing the polyphenols-proteins interaction. Besides, the percentage of interaction between milk protein fractions with PIC was mentioned using HPLC technique.

Orijinal dilİngilizce
Makale numarası100558
DergiSurfaces and Interfaces
Hacim20
DOI'lar
Yayın durumuYayınlandı - Eyl 2020

Bibliyografik not

Publisher Copyright:
© 2020 Elsevier B.V.

Finansman

The authors express their gratitude to The Scientific and Technological Research Council of Turkey (TÜBİTAK). I would like to acknowledgment everyone support me to do this research, specially Dr. Fatih Bildik doctor of Istanbul Technical University, Chemistry and Metallurgical Faculty, Food Engineering Department.

FinansörlerFinansör numarası
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu
Istanbul Teknik Üniversitesi

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