Solid-phase extraction spectrophotometric determination of total antioxidant capacity in antioxidant-poor samples by using the ferric-ferrozine method

Kadriye Isil Berker, Dilek Ozyurt, Birsen Demirata, Resat Apak*

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: Dergiye katkıMakalebilirkişi

2 Atıf (Scopus)

Özet

Ferrozine (FZ) preferentially stabilizes Fe(II) over Fe(III) to raise the ferric reduction potential and oxidize antioxidants. The advantages of the ferric-ferrozine method over other iron-based total antioxidant capacity assays were: (i) higher molar absorptivity and enhanced sensitivity, (ii) lower interference from foreign ions, (iii) wide pH tolerance (iv) additivity of the absorbances for mixtures. Solid-phase extraction (SPE) could be combined with spectrophotometry, because the magenta-colored anionic Fe(II)-FZ complex was quantitatively sorbed on Sephadex QAE A-25 resin. The sensitivity enhancement using the resin enabled us to conduct total antioxidant capacity (TAC) measurements of antioxidant-poor samples. The apparent molar absorptivity, linear concentration range and trolox equivalent antioxidant capacities (TEAC) of certain antioxidants were found. The calibration curves (lines) of trolox, rutin, and rosmarinic acid individually and in herbal infusions-by using the method of standard additions-were parallel, confirming that the added antioxidants did not interact with herbal constituents to cause chemical deviations from Beer's law.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)683-689
Sayfa sayısı7
DergiAnalytical Sciences
Hacim33
Basın numarası6
DOI'lar
Yayın durumuYayınlandı - 2017

Bibliyografik not

Publisher Copyright:
© 2017 The Japan Society for Analytical Chemistry.

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