Sequence specific recognition of ssDNA by fluorophore 3-hydroxyflavone

Asli Capan; Muge S. Bostan; Erkan Mozioglu; Muslum Akoz; Ahmet C. Goren; Mehmet S. Eroglu; Turan Ozturk

doi:10.1016/j.jphotobiol.2015.10.019

Sequence specific recognition of ssDNA by fluorophore 3-hydroxyflavone

Asli Capan, Muge S. Bostan, Erkan Mozioglu, Muslum Akoz, Ahmet C. Goren, Mehmet S. Eroglu^*, Turan Ozturk

^*Bu çalışma için yazışmadan sorumlu yazar

Kimya Bölümü

Araştırma sonucu: ???type-name??? › Makale › bilirkişi

5 Atıf (Scopus)

Özet

A fully water soluble 3-hydroxyflavone (3HF) derivative, N-(3-hydroxy-4′-flavonyl)-N,N,N-trimethylammonium sulfate (3HFNMe3) was synthesized. Investigation of its emissions at varying wavelengths revealed that it had three emission bands of normal (N∗), anionic (A∗) and tautomeric (T∗), in ultrapure water. Recognition of single-stranded ten ssDNA chains, having different nucleotide sequences was studied, using the ratiometric change of the intensities of the two bands (A∗/T∗), depending upon the varying environment of the 3HFNMe3 with different ssDNA chains. Addition of the ssDNA chains to the 3HFNMe3 solution caused gradual quenching of the A∗ band and had almost no effect on the T∗ band. As the ratios of the two bands (A∗/T∗) vs increasing amount of the ssDNAs generated characteristic curves for each ssDNA chain, it became possible to identify the chains with their characteristic curves.

Orijinal dil	İngilizce
Sayfa (başlangıç-bitiş)	391-396
Sayfa sayısı	6
Dergi	Journal of Photochemistry and Photobiology B: Biology
Hacim	153
DOI'lar	https://doi.org/10.1016/j.jphotobiol.2015.10.019
Yayın durumu	Yayınlandı - Ara 2015

Bibliyografik not

Publisher Copyright:
© 2015 Elsevier B.V.

Finansman

We thank Dr. Andrey Klymchenko of University of Strasbourg for his excellent successions on anionic mechanism, Dr. Mehmet Emin Cinar for useful discussions on computational calculations, and the Scientific and Technological Research Council of Turkey ( 104T081 ), Istanbul Technical University and Unsped Global Lojistik for financial supports. We also thank National Center for High Performance Computing of Turkey (UYBHM) for computing resources.

Finansörler	Finansör numarası
Istanbul Technical University and Unsped Global Lojistik
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu	104T081

Belgeye Erişim

10.1016/j.jphotobiol.2015.10.019

Diğer Dosyalar ve Linkler

Link to publication in Scopus

Alıntı Yap

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title = "Sequence specific recognition of ssDNA by fluorophore 3-hydroxyflavone",

abstract = "A fully water soluble 3-hydroxyflavone (3HF) derivative, N-(3-hydroxy-4′-flavonyl)-N,N,N-trimethylammonium sulfate (3HFNMe3) was synthesized. Investigation of its emissions at varying wavelengths revealed that it had three emission bands of normal (N∗), anionic (A∗) and tautomeric (T∗), in ultrapure water. Recognition of single-stranded ten ssDNA chains, having different nucleotide sequences was studied, using the ratiometric change of the intensities of the two bands (A∗/T∗), depending upon the varying environment of the 3HFNMe3 with different ssDNA chains. Addition of the ssDNA chains to the 3HFNMe3 solution caused gradual quenching of the A∗ band and had almost no effect on the T∗ band. As the ratios of the two bands (A∗/T∗) vs increasing amount of the ssDNAs generated characteristic curves for each ssDNA chain, it became possible to identify the chains with their characteristic curves.",

keywords = "3-Hydroxyflavone, DNA, Fluorophore, Specific recognition, Spectroscopic methods",

author = "Asli Capan and Bostan, {Muge S.} and Erkan Mozioglu and Muslum Akoz and Goren, {Ahmet C.} and Eroglu, {Mehmet S.} and Turan Ozturk",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

year = "2015",

month = dec,

doi = "10.1016/j.jphotobiol.2015.10.019",

language = "English",

volume = "153",

pages = "391--396",

journal = "Journal of Photochemistry and Photobiology B: Biology",

issn = "1011-1344",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Sequence specific recognition of ssDNA by fluorophore 3-hydroxyflavone

AU - Capan, Asli

AU - Bostan, Muge S.

AU - Mozioglu, Erkan

AU - Akoz, Muslum

AU - Goren, Ahmet C.

AU - Eroglu, Mehmet S.

AU - Ozturk, Turan

PY - 2015/12

Y1 - 2015/12

N2 - A fully water soluble 3-hydroxyflavone (3HF) derivative, N-(3-hydroxy-4′-flavonyl)-N,N,N-trimethylammonium sulfate (3HFNMe3) was synthesized. Investigation of its emissions at varying wavelengths revealed that it had three emission bands of normal (N∗), anionic (A∗) and tautomeric (T∗), in ultrapure water. Recognition of single-stranded ten ssDNA chains, having different nucleotide sequences was studied, using the ratiometric change of the intensities of the two bands (A∗/T∗), depending upon the varying environment of the 3HFNMe3 with different ssDNA chains. Addition of the ssDNA chains to the 3HFNMe3 solution caused gradual quenching of the A∗ band and had almost no effect on the T∗ band. As the ratios of the two bands (A∗/T∗) vs increasing amount of the ssDNAs generated characteristic curves for each ssDNA chain, it became possible to identify the chains with their characteristic curves.

AB - A fully water soluble 3-hydroxyflavone (3HF) derivative, N-(3-hydroxy-4′-flavonyl)-N,N,N-trimethylammonium sulfate (3HFNMe3) was synthesized. Investigation of its emissions at varying wavelengths revealed that it had three emission bands of normal (N∗), anionic (A∗) and tautomeric (T∗), in ultrapure water. Recognition of single-stranded ten ssDNA chains, having different nucleotide sequences was studied, using the ratiometric change of the intensities of the two bands (A∗/T∗), depending upon the varying environment of the 3HFNMe3 with different ssDNA chains. Addition of the ssDNA chains to the 3HFNMe3 solution caused gradual quenching of the A∗ band and had almost no effect on the T∗ band. As the ratios of the two bands (A∗/T∗) vs increasing amount of the ssDNAs generated characteristic curves for each ssDNA chain, it became possible to identify the chains with their characteristic curves.

KW - 3-Hydroxyflavone

KW - DNA

KW - Fluorophore

KW - Specific recognition

KW - Spectroscopic methods

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U2 - 10.1016/j.jphotobiol.2015.10.019

DO - 10.1016/j.jphotobiol.2015.10.019

M3 - Article

C2 - 26555642

AN - SCOPUS:84946562261

SN - 1011-1344

VL - 153

SP - 391

EP - 396

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

ER -

Sequence specific recognition of ssDNA by fluorophore 3-hydroxyflavone

Özet

Bibliyografik not

Finansman

Belgeye Erişim

Diğer Dosyalar ve Linkler

Parmak izi

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