Selective Electrochemical Determination of Dopamine with Molecularly Imprinted Poly(Carbazole-co-Aniline) Electrode Decorated with Gold Nanoparticles

Şener Sağlam, Aysu Arman, Ayşem Üzer, Belkıs Ustamehmetoğlu, Esma Sezer, Reşat Apak*

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16 Atıf (Scopus)

Özet

Dopamine (DA) is a significant neurotransmitter in the central nervous system, coexisting with uric acid (UA) and ascorbic acid (AA). UA and AA are easily oxidizable compounds having potentials close to that of DA for electrochemical analysis, resulting in overlapping voltammetric response. In this work, a novel molecularly imprinted (MI) electrochemical sensor was proposed for selective determination of DA (in the presence of up to 80-fold excess of UA and AA), relying on gold nanoparticles (Aunano)-decorated glassy carbon (GC) electrode coated with poly(carbazole (Cz)-co-aniline (ANI)) copolymer film incorporating DA as template (DA imprinted-GC/P(Cz-co-ANI)-Aunano electrode, DA-MIP-Aunano electrode). The DA recognizing sensor electrode showed great electroactivity for analyte oxidation in 0.2 mol L−1 pH 7 phosphate buffer. Square wave voltammetry (SWV) was performed within 10−4–10−5 mol L−1 of DA, of which the oxidation peak potential was observed at 0.16 V. The limit of detection (LOD) and limit of quantification (LOQ) were 2.0×10−6 and 6.7×10−6 mol L−1, respectively. Binary and ternary synthetic mixtures of DA-UA, DA-AA and DA-UA-AA yielded excellent recoveries for DA. Additionally, DA was quantitatively recovered from a real sample of bovine serum spiked with DA, and determined in concentrated dopamine injection solution. The developed SWV method was statistically validated against a literature potentiodynamic method using a caffeic acid modified-GC electrode.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)964-970
Sayfa sayısı7
DergiElectroanalysis
Hacim32
Basın numarası5
DOI'lar
Yayın durumuYayınlandı - 1 May 2020

Bibliyografik not

Publisher Copyright:
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Finansman

Dr. Apak thanks Istanbul University-Cerrahpasa Research Fund for the support given to BEK-Project-2017-24173, enabling him to participate “Pittcon Conference 2017” in Chacago,USA. Dr. Apak thanks Istanbul University‐Cerrahpasa Research Fund for the support given to BEK‐Project‐2017‐24173, enabling him to participate “Pittcon Conference 2017” in Chacago,USA.

FinansörlerFinansör numarası
Istanbul University-Cerrahpasa Research Fund
Istanbul University‐Cerrahpasa Research Fund

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