Saccharification yield through enzymatic hydrolysis of the steam-exploded pinewood

Merve Nazli Borand*, Asli Isler Kaya, Filiz Karaosmanoglu

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12 Atıf (Scopus)

Özet

Pressure, temperature, and retention time are the most studied parameters in steam explosion pretreatment. However, this work aimed to fix these parameters and to evaluate the influences of several less investigated steam explosion parameters on the saccharification yield in hydrolysis. In this study, firstly, pinewood samples smaller than 200 μm were treated with steam explosion at 190 °C for 10 min. The variable parameters were biomass loading, N2 pressure, and release time. Steam-exploded samples were hydrolyzed with the Trichoderma reesei enzyme for saccharification for 72 h. The sugar content of the resultant products was analyzed to estimate the yield of sugars (such as glucose, xylose, galactose, mannose, and arabinose). The best glucose yield in the pulp was achieved with 4 g of sample, N2 pressure of 0.44 MPa, and short release time (22 s). These conditions gave a glucose yield of 97.72% in the pulp, and the xylose, mannose, galactose, and arabinose yields in the liquid fraction were found to be 85.59%, 87.76%, 86.43%, and 90.3%, respectively.

Orijinal dilİngilizce
Makale numarasıen13174552
DergiEnergies
Hacim13
Basın numarası17
DOI'lar
Yayın durumuYayınlandı - Eyl 2020

Bibliyografik not

Publisher Copyright:
© 2020 by the authors.

Finansman

Acknowledgments: The Turkish Council of Higher Education supported this research under the International Research Scholarship Program. The experiments were performed in James Martin Laboratory in the Department of Biological and Agricultural Engineering at the University of Idaho in the USA. The authors gratefully acknowledge support from Jon Van Gerpen and the personnel of James Martin Laboratory.

FinansörlerFinansör numarası
James Martin Laboratory
Turkish Council of Higher Education

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