Pyrolidium dithiocarbamate prevents shockwave lithotripsy-induced renal injury through inhibition of nuclear factor-kappa B and inducible nitric oxide synthase activity in rats

Vulcan Tugcu*, Muzaffer Bas, Emin Ozbeck, Emay Kemahli, Yasar Volkan Arinci, Mehmet Tuhri, Tuncay Altug, Ali Ihsan Tasci

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: Dergiye katkıMakalebilirkişi

10 Atıf (Scopus)

Özet

Purpose: Extracorporeal shockwave lithotripsy (SWL) is commonly used for treatment of renal stones. Free oxygen radicals are involved in the pathophysiology of renal injury due to SWL. We investigated the protective effect of pyrolidium dithiocarbamate (PDTC), an antioxidant and nuclear factor κ-B (NFκ-B) inhibitor, against renal injury. Materials and Methods: Forty-eight rats were divided into three groups group 1, controls; group 2, SWL (15 kW, 1500 pulses); and group 3, SWL + pyrolidium dithiocarbamate (PDTC) (100 mg/kg/day given intraperitoneally 1 day before and 5 days after SWL). The rats were sacrificed and their kidneys were removed on days 7 and 35 after SWL. Samples were fixed in formaldehyde solution, and renal tissues were examined for proximal tubular injury under light microscopy. iNOS activity and active subunit of NFκ-B, p65, were evaluated immunohistochemically using rat monoclonal antibodies interpreting results semiquantitatively. Results: There were significant differences between SWL and control groups on days 7 and 35, considering histological changes under light microscope (p < 0.02). There was significant decrease in necrosis and fibrosis in PDTC group compared to SWL group. Expression of inducible nitric oxide synthase (iNOS) and p65 on days 7 and 35 were at basal levels as seen with immunohistochemical staining. There were high concentrations of iNOS and p65 in the SWL group (P < 0.02). No significant difference in concentrations was seen between the control and the PDTC groups (P > 0.02). Conclusion: We found that curcumin decreased the expression of iNOS, p65, and serum nitric oxide levels, and helped prevent interstitial, glomerular, tubular epithelial, and endothelial cellular injury. We believe that PDTC could be used, particularly in high-risk patients, as a protective agent against renal injury due to SWL.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)559-566
Sayfa sayısı8
DergiJournal of Endourology
Hacim22
Basın numarası3
DOI'lar
Yayın durumuYayınlandı - 1 Mar 2008

Parmak izi

Pyrolidium dithiocarbamate prevents shockwave lithotripsy-induced renal injury through inhibition of nuclear factor-kappa B and inducible nitric oxide synthase activity in rats' araştırma başlıklarına git. Birlikte benzersiz bir parmak izi oluştururlar.

Alıntı Yap