Production of structured lipids containing conjugated linolenic acid: Optimisation by response surface methodology

Begum Elibal, H. Funda Suzen, H. Ayse Aksoy*, Guldem Ustun, Melek Tuter

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Özet

Structured lipids containing conjugated linolenic acid (CLNA) were produced separately by enzymatic acidolysis reaction of corn and canola oils (CAO) with bitter gourd (Momordica charantia L) seed oil fatty acids [bitter gourd seed oil fatty acids (BGFA)]. Reactions were conducted using a commercial immobilised sn-1,3-specific lipase from Thermomyces lanuginosa (Lipozyme TL IM) in hexane. The effects of reaction time, substrate molar ratio, temperature and enzyme amount on incorporation yield of CLNA were investigated and optimised by response surface methodology with three-level, two-factor face-centred cube design. When reactions were conducted using 10% enzyme for 3h, the optimum reaction conditions were found for corn oil (CO) as 53.5°C and 5.9:1 BGFA/CO molar ratio. At these conditions, the incorporation of CLNA into CO was determined as 41.4%. However, CLNA incorporation into CAO was resulted as 37% at optimum conditions which were 54.2°C and 6.8:1 BGFA/CAO molar ratio.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)1422-1427
Sayfa sayısı6
DergiInternational Journal of Food Science and Technology
Hacim46
Basın numarası7
DOI'lar
Yayın durumuYayınlandı - Tem 2011

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