Preparation and characterization of sulfonyl-hydrazine attached poly(styrene-divinylbenzene) beads for separation of albumin

Novel sulfonyl-hydrazine carrying poly(styrene-divinylbenzene), poly(S-DVB)-S-NH₂ ion-exchange beads (size between 210 and 425 μm) were prepared via suspension polymerization and, were first used as an ion-exchange support for adsorption of human serum albumin (HSA) from aqueous solution. The influence of pH, equilibrium time, ionic strength and initial albumin concentration on the adsorption capacity of the poly(S-DVB)-S-NH₂ ion-exchange beads has been investigated in a batch system and the unmodified poly(S-DVB) beads were used as control system. Maximum HSA adsorption onto poly(S-DVB)-S-NH₂ ion-exchange beads was found to be 63.05 mg/g at pH 7.0. The experimental equilibrium data for HSA adsorption onto poly(S-DVB)-S-NH₂ beads was well described by the Langmuir isotherm model. Kinetic parameters of this adsorption system were also analyzed for HSA adsorption onto beads and, the first-order rate equations were favorable. Finally, the poly(S-DVB)-S-NH₂ beads was used for the purification of HSA from whole human serum and, the purity of the eluted HSA from the beads was determined as 89% by HPLC from single step purification protocol.