Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation

Muhammet U. Kahveci; Mustafa Ciftci; Serap Evran; Suna Timur; Yusuf Yagci

doi:10.1080/15685551.2014.971392

Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation

Muhammet U. Kahveci, Mustafa Ciftci, Serap Evran, Suna Timur, Yusuf Yagci^*

^*Bu çalışma için yazışmadan sorumlu yazar

Kimya Bölümü

Araştırma sonucu: Dergiye katkı › Makale › bilirkişi

12 Atıf (Scopus)

Özet

A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.

Orijinal dil	İngilizce
Sayfa (başlangıç-bitiş)	129-136
Sayfa sayısı	8
Dergi	Designed Monomers and Polymers
Hacim	18
Basın numarası	2
DOI'lar	https://doi.org/10.1080/15685551.2014.971392
Yayın durumu	Yayınlandı - 17 Şub 2015

Bibliyografik not

Publisher Copyright:
© 2014 Taylor & Francis.

Finansman

The authors are thankful for Istanbul Technical University Research Fund and TUBITAK (The Scientific and Technical Research Council of Turkey) Project No. 113Z234. M.C. thanks TUBITAK for the financial support by means of a graduate program.

Finansörler	Finansör numarası
Istanbul Technical University Research Fund
TUBITAK
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu	113Z234

Belgeye Erişim

10.1080/15685551.2014.971392

Diğer Dosyalar ve Linkler

Link to publication in Scopus

Alıntı Yap

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title = "Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation",

abstract = "A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.",

keywords = "bioconjugation, click chemistry, hydrogels, photopolymerization, protein adsorption",

author = "Kahveci, {Muhammet U.} and Mustafa Ciftci and Serap Evran and Suna Timur and Yusuf Yagci",

note = "Publisher Copyright: {\textcopyright} 2014 Taylor & Francis.",

year = "2015",

month = feb,

day = "17",

doi = "10.1080/15685551.2014.971392",

language = "English",

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TY - JOUR

T1 - Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation

AU - Kahveci, Muhammet U.

AU - Ciftci, Mustafa

AU - Evran, Serap

AU - Timur, Suna

AU - Yagci, Yusuf

PY - 2015/2/17

Y1 - 2015/2/17

N2 - A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.

AB - A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.

KW - bioconjugation

KW - click chemistry

KW - hydrogels

KW - photopolymerization

KW - protein adsorption

UR - http://www.scopus.com/inward/record.url?scp=84916210923&partnerID=8YFLogxK

U2 - 10.1080/15685551.2014.971392

DO - 10.1080/15685551.2014.971392

M3 - Article

AN - SCOPUS:84916210923

SN - 1385-772X

VL - 18

SP - 129

EP - 136

JO - Designed Monomers and Polymers

JF - Designed Monomers and Polymers

IS - 2

ER -

Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation

Özet

Bibliyografik not

Finansman

Belgeye Erişim

Diğer Dosyalar ve Linkler

Parmak izi

Alıntı Yap