Partial purification of Nigella sativa L. seed lipase and its application in hydrolytic reactions. Enrichment of γ-linolenic acid from borage oil

Melek Tuter, H. Ayşe Aksoy*, Guldem Ustun, Sergio Riva, Francesco Secundo, Serhat Ipekler

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: ???type-name???Makalebilirkişi

4 Atıf (Scopus)

Özet

Selective hydrolysis of borage (Borago officinalis L.) oil was catalyzed by two lipase preparations of Nigella sativa L. seeds at 40°C in a mixture of borage oil, water, and hexane. Ammonium sulfate-precipitated lipase (Nigella PL) and lipase partially purified by DEAE-ion exchange chromatography (Nigella CPL) exhibited a negative specificity toward γ-linolenic acid (GLA). Best results were obtained in the experiments conducted with 330 U/g oil of Nigella PL and 200 U/g oil of Nigella CPL. When 330 U/g oil of Nigella PL was used, after 8 h the GLA level rose from 21.9% in the starting oil to 29.6 and 41.8% in TAG and DAG fractions of the product mixtures, respectively (1.5-fold enrichment of GLA in the total unhydrolyzed acylglycerol fraction). At 200 U/g oil enzyme concentration of Nigella CPL, after 77 h maximum GLA enrichment was observed in the DAG fraction. The GLA content of the DAG increased to 34.6%, corresponding to almost 1.6-fold enrichment. The relative inability of Nigella sativa lipase(s) to hydrolyze γ-linolenoyl moieties of TAG can be used for the enrichment of this acid in the unhydrolyzed acylglycerol fractions of GLA-containing oils.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)237-241
Sayfa sayısı5
DergiJAOCS, Journal of the American Oil Chemists' Society
Hacim80
Basın numarası3
DOI'lar
Yayın durumuYayınlandı - Mar 2003

Finansman

This work was supported in part by joint research project grants from CNR (Consiglio Nazionale delle Ricerche, Italy) and TUBITAK (Turkish Scientific and Technical Research Center). Ay¸segül Ki-rayog˘lu and Ilker Demiryol provided technical assistance.

FinansörlerFinansör numarası
TUBITAK
Turkish Scientific and Technical Research Center
Consiglio Nazionale delle Ricerche

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