Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay

Muhammad Mujtaba; Bahar Akyuz Yilmaz; Demet Cansaran-Duman; Lalehan Akyuz; Sevcan Yangın; Murat Kaya; Talip Çeter; Khalid Mahmood Khawar

doi:10.1007/s00289-020-03531-7

Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay

Muhammad Mujtaba^*
, Bahar Akyuz Yilmaz
, Demet Cansaran-Duman
, Lalehan Akyuz
, Sevcan Yangın
, Murat Kaya
, Talip Çeter
, Khalid Mahmood Khawar

^*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: Dergiye katkı › Makale › bilirkişi

5 Atıf (Scopus)

Özet

Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.

Orijinal dil	İngilizce
Sayfa (başlangıç-bitiş)	519-540
Sayfa sayısı	22
Dergi	Polymer Bulletin
Hacim	79
Basın numarası	1
DOI'lar	https://doi.org/10.1007/s00289-020-03531-7
Yayın durumu	Yayınlandı - Oca 2022
Harici olarak yayınlandı	Evet

Bibliyografik not

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.

Finansman

MM would like to thanks the scientific and technological council of Turkey (TÜBİTAK) for supporting his thesis under program number TÜBİTAK-2215. The authors would like to acknowledge the technical and human support provided by Biotechnology Institute, Ankara University, and Scientific and Technological Application and Research Center, Aksaray University, Turkey. MM would like to thanks the scientific and technological council of Turkey (T?B?TAK) for supporting his thesis under program number T?B?TAK-2215. The authors would like to acknowledge the technical and human support provided by Biotechnology Institute, Ankara University, and Scientific and Technological Application and Research Center, Aksaray University, Turkey.

Finansörler	Finansör numarası
Ankara University, and Scientific and Technological Application and Research Center
Ankara Universitesi Biyoteknoloji Enstitusu
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu	TÜBİTAK-2215
Aksaray Üniversitesi
Scientific and Technological Council of Turkey

BM SKH

Bu sonuç, aşağıdaki Sürdürülebilir Kalkınma Hedefine/Hedeflerine katkıda bulunur

SKH 3 Sağlık ve Kaliteli Yaşam

Belgeye Erişim

10.1007/s00289-020-03531-7

Diğer Dosyalar ve Linkler

Link to publication in Scopus

Alıntı Yap

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title = "Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay",

abstract = "Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.",

keywords = "Controlled-drug release, Real-time cell analyzer, Sporopollenin, xCELLigence",

author = "Muhammad Mujtaba and Yilmaz, \{Bahar Akyuz\} and Demet Cansaran-Duman and Lalehan Akyuz and Sevcan Yangın and Murat Kaya and Talip {\c C}eter and Khawar, \{Khalid Mahmood\}",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.",

year = "2022",

month = jan,

doi = "10.1007/s00289-020-03531-7",

language = "English",

volume = "79",

pages = "519--540",

journal = "Polymer Bulletin",

issn = "0170-0839",

publisher = "Springer Verlag",

number = "1",

}

TY - JOUR

T1 - Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay

AU - Mujtaba, Muhammad

AU - Yilmaz, Bahar Akyuz

AU - Cansaran-Duman, Demet

AU - Akyuz, Lalehan

AU - Yangın, Sevcan

AU - Kaya, Murat

AU - Çeter, Talip

AU - Khawar, Khalid Mahmood

PY - 2022/1

Y1 - 2022/1

N2 - Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.

AB - Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.

KW - Controlled-drug release

KW - Real-time cell analyzer

KW - Sporopollenin

KW - xCELLigence

UR - https://www.scopus.com/pages/publications/85098644215

U2 - 10.1007/s00289-020-03531-7

DO - 10.1007/s00289-020-03531-7

M3 - Article

AN - SCOPUS:85098644215

SN - 0170-0839

VL - 79

SP - 519

EP - 540

JO - Polymer Bulletin

JF - Polymer Bulletin

IS - 1

ER -

Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay

Özet

Bibliyografik not

Finansman

BM SKH

Belgeye Erişim

Diğer Dosyalar ve Linkler

Parmak izi

Alıntı Yap