Microfluidic device on a nonwoven fabric: A potential biosensor for lactate detection

Gülçin Baysal, Sakip Önder, İkilem Göcek, Levent Trabzon, Hüseyin Kızıl, Fatma Neşe Kök, Burçak Karagüzel Kayaoğlu

Araştırma sonucu: ???type-name???Makalebilirkişi

35 Atıf (Scopus)

Özet

In the present study, a novel, wearable textile based microfluidic device was developed that provides a non-invasive, rapid, semi-quantitative detection of the lactate level in simulated sweat solution. The potential application was envisioned to be a biosensor that can monitor an athlete's physical status during exercise. A photolithography technique was used for the fabrication of hydrophilic micro channels and reservoirs surrounded by hydrophobic barriers made from SU-8 negative photoresist. The reservoirs were functionalized by co-immobilization of lactate oxidase (LOX) and horseradish peroxidase (POX) enzymes. LOX uses L-(+)-Lactic acid as substrate and produces H2O2 which is a POX substrate. Then, POX oxidases H2O2 in the presence of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) and results in color formation. The studies showed that excess amount of analyte presence resulted in analyte inhibition. It was also shown that analyte pH and temperature were effective on the color formation. For effective results, analyte pH and temperature should be ≥5℃ and 25–30℃, respectively. Lower pH and higher temperature values resulted in a decrease in the enzyme activity. The textile based biosensor system could make a semi-quantitative visual detection to differentiate between the normal (<5 mM) and high (≥5 mM) lactate level: while a high lactate level led to a denser purple color formation, normal levels led to a light purple formation and a green color started to be observed.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)1729-1741
Sayfa sayısı13
DergiTextile Reseach Journal
Hacim84
Basın numarası16
DOI'lar
Yayın durumuYayınlandı - Eki 2014

Finansman

This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (grant number 111M483).

FinansörlerFinansör numarası
TUBITAK111M483
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu

    Parmak izi

    Microfluidic device on a nonwoven fabric: A potential biosensor for lactate detection' araştırma başlıklarına git. Birlikte benzersiz bir parmak izi oluştururlar.

    Alıntı Yap