Live Cell Imaging of Peptide Uptake Using a Microfluidic Platform

Merve Yuce, Elif Ozkirimli, Berna Sariyar Akbulut, Kutlu Ulgen*

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: Dergiye katkıMakalebilirkişi

Özet

Cell penetrating peptides (CPPs) are unique molecules with the ability to pass through biological membranes as they carry their cargoes into the cells. Endocytosis and direct penetration have been proposed as the two major mechanisms for their internalization. However, the involvement of oligo peptide permease (Opp) in bacterial CPP uptake is an issue that yet remains to be elucidated. Here, a microfluidic platform coupled with live cell imaging was used to monitor CPP uptake in Escherichia coli. The set-up enabled imaging on a single-cell basis, helped to capture cell dynamics at a micro-scale and provided quantitative real-time information. The constructed platform also provided evidence on the contribution of Opp to peptide uptake. Additional high-resolution visualization under confocal microscopy confirmed that in the absence of Opp, the internalized peptides accumulated in the periplasm. Here, we have shown that microfluidic platforms, which have successful applications in different areas, can be used as a highly reliable and versatile tool for studying peptide uptake in bacterial cells.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)2003-2013
Sayfa sayısı11
DergiInternational Journal of Peptide Research and Therapeutics
Hacim27
Basın numarası3
DOI'lar
Yayın durumuYayınlandı - Eyl 2021

Bibliyografik not

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature B.V.

Parmak izi

Live Cell Imaging of Peptide Uptake Using a Microfluidic Platform' araştırma başlıklarına git. Birlikte benzersiz bir parmak izi oluştururlar.

Alıntı Yap