Isolation Of Colour Component From italicJuglans Regia L. Chromatographic Determination, Spectrophotometric Investigation And Dyeing Properties

In this paper, we present a more sensitive quantification of the high performance liquid chromatographic (HPLC) method to analyze juglon in madder root (Juglans regia L. or walnut) which appears in the South of Anatolia. The juglon is separated on a Zorbax Eclipse XDB C18 column with a phosphoric acid-acetonitrile gradient as eluent and measured with UV detection at 250 nm. With this method, the juglon can be analyzed. The regression equation that is obtained from the calibration curve reveals a linear relationship (r = 0.9999) between the mass of the juglon injected and the peak area. Then, the color component responsible for dyeing is determined and its chemical constituents are established based on chemical and spectroscopic investigations. Afterwards, the wool fabrics are dyed by using pre, together and last mordanting, and mordantless techniques. Fastness to light, washing and rubbing of the dyed fabrics are measured and discussed.