Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography

Nevin Öztekin; Selda Başkan; S. Evrim Kepekçi; F. Bedia Erim; Gülaçti Topçu

doi:10.1016/j.jpba.2009.04.012

Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography

Nevin Öztekin
, Selda Başkan
, S. Evrim Kepekçi
, F. Bedia Erim^*
, Gülaçti Topçu

^*Bu çalışma için yazışmadan sorumlu yazar

Kimya Bölümü

Istanbul Technical University
Istanbul University

Araştırma sonucu: Dergiye katkı › Makale › bilirkişi

29 Atıf (Scopus)

Özet

In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N = 3) were 3.269 and 4.518 μg/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts.

Orijinal dil	İngilizce
Sayfa (başlangıç-bitiş)	439-442
Sayfa sayısı	4
Dergi	Journal of Pharmaceutical and Biomedical Analysis
Hacim	51
Basın numarası	2
DOI'lar	https://doi.org/10.1016/j.jpba.2009.04.012
Yayın durumu	Yayınlandı - 20 Oca 2010

Finansman

We thank the TUBITAK (Grant TBA6-2312, 103T053) for financial support.

Finansörler	Finansör numarası
TUBITAK	103T053, TBA6-2312

Belgeye Erişim

10.1016/j.jpba.2009.04.012

Diğer Dosyalar ve Linkler

Link to publication in Scopus

Alıntı Yap

@article{01cf3603e7e048208acba4b1e452c0a6,

title = "Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography",

abstract = "In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia species. The optimal separation electrolyte was 50 mmol/L SDS and 25\% methanol at pH 11.5. The limits of detection (S/N = 3) were 3.269 and 4.518 μg/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts.",

keywords = "7-O-acetylhorminone, Capillary electrophoresis, Diterpenoids, Horminone, MEKC, Plant, Salvia",

author = "Nevin {\"O}ztekin and Selda Ba{\c s}kan and \{Evrim Kepek{\c c}i\}, S. and Erim, \{F. Bedia\} and G{\"u}la{\c c}ti Top{\c c}u",

year = "2010",

month = jan,

day = "20",

doi = "10.1016/j.jpba.2009.04.012",

language = "English",

volume = "51",

pages = "439--442",

journal = "Journal of Pharmaceutical and Biomedical Analysis",

issn = "0731-7085",

publisher = "Elsevier B.V.",

number = "2",

}

Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography. / Öztekin, Nevin; Başkan, Selda; Evrim Kepekçi, S. et al.
In: Journal of Pharmaceutical and Biomedical Analysis, Hac. 51, No. 2, 20.01.2010, p. 439-442.

Araştırma sonucu: Dergiye katkı › Makale › bilirkişi

TY - JOUR

T1 - Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography

AU - Öztekin, Nevin

AU - Başkan, Selda

AU - Evrim Kepekçi, S.

AU - Erim, F. Bedia

AU - Topçu, Gülaçti

PY - 2010/1/20

Y1 - 2010/1/20

N2 - In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N = 3) were 3.269 and 4.518 μg/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts.

AB - In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N = 3) were 3.269 and 4.518 μg/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts.

KW - 7-O-acetylhorminone

KW - Capillary electrophoresis

KW - Diterpenoids

KW - Horminone

KW - MEKC

KW - Plant

KW - Salvia

UR - https://www.scopus.com/pages/publications/70349829857

U2 - 10.1016/j.jpba.2009.04.012

DO - 10.1016/j.jpba.2009.04.012

M3 - Article

C2 - 19446421

AN - SCOPUS:70349829857

SN - 0731-7085

VL - 51

SP - 439

EP - 442

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

IS - 2

ER -

Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography

Özet

Finansman

Belgeye Erişim

Diğer Dosyalar ve Linkler

Parmak izi

Alıntı Yap