Inhibition effect of isopropanol on acetyl-CoA synthetase expression level of acetoclastic methanogen, Methanosaeta concilii

Bahar Ince, Gozde Koksel, Zeynep Cetecioglu, Nilgun Ayman Oz, Halil Coban*, Orhan Ince

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: Dergiye katkıMakalebilirkişi

25 Atıf (Scopus)

Özet

Isopropanol is a widely found solvent in industrial wastewaters, which have commonly been treated using anaerobic systems. In this study, inhibitory effect of isopropanol on the key microbial group in anaerobic bioreactors, acetoclastic methanogens, was investigated. Anaerobic sludges in serum bottles were repeatedly fed with acetate and isopropanol; and quantitative real-time PCR was used for determining effect of isopropanol on the expression level of a key enzyme in acetoclastic methane production, acetyl-CoA synthetase of Methanosaeta concilii. Active Methanosaeta spp. cells were also quantified using Fluorescent in situ hybridization (FISH). Transcript abundance of acetyl-CoA synthetase was 1.23±0.62×10 6mRNAs/mL in the uninhibited reactors with 222mL cumulative methane production. First exposure to isopropanol resulted in 71.2%, 84.7%, 89.2% and 94.6% decrease in mRNA level and 35.0%, 65.0%, 91.5% and 100.0% reduction in methane production for isopropanol concentrations of 0.1M, 0.5M, 1.0M and 2.0M, respectively. Repeated exposures resulted in higher inhibitions; and at the end of test, fluorescent intensities of active Methanosaeta cells were significantly decreased due to isopropanol. The overall results indicated that isopropanol has an inhibitory effect on acetoclastic methanogenesis; and the inhibition can be detected by monitoring level of acetyl-CoA transcripts and rRNA level.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)95-99
Sayfa sayısı5
DergiJournal of Biotechnology
Hacim156
Basın numarası2
DOI'lar
Yayın durumuYayınlandı - 10 Kas 2011

Finansman

This work was supported by grants from the Foundation of the Scientific and Technological Research Council of Turkey (Project No. 106Y241), and Research Fund of Bogazici University (Project No. 01M102).

FinansörlerFinansör numarası
Foundation of the Scientific and Technological Research Council of Turkey106Y241
Research Fund of Bogazici University01M102

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