Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species

Tercüme edilen katkı başlığı: Endemik tıbbi tür Digitalis lamarckii Ivan.'ın kotiledon yapraǧı segmentlerinden indirekt somatik embriyo ve sürgün oluşumu

Sandeep Kumar Verma, Bahtiyar Buhara Yücesan, Songül Gürel, Ekrem Gürel*

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: Dergiye katkıMakalebilirkişi

22 Atıf (Scopus)

Özet

The is study describes, for the first time, an in vitro protocol for the development of somatic embryos and shoots from a callus derived from cotyledonary leaf segments excised from 3-week-old in vitro-germinated seedlings of Digitalis lamarckii Ivan. (dwarf foxglove), an endemic medicinal species of Turkey. The embryogenic callus was induced on Murashige and Skoog (MS) medium containing 0.54 μM α-naphthalene acetic acid (NAA) and 2.22 μM 6-benzylaminopurine (BAP). Somatic embryos developed readily when the embryogenic callus was transferred to MS medium containing BAP (4.44 or 8.87 μM) alone or BAP combined with NAA (1.34, 2.69, or 5.37 μM). The most effective hormonal combination for somatic embryogenesis was 1.34 μM NAA and 8.87 μM BAP, which produced a mean of 37.0 embryos per cotyledonary leaf explant. An organogenic callus was induced on MS medium containing 2.69 μM NAA and 2.22 μM BAP. Shoot development was observed when the organogenic callus was transferred to MS medium containing different concentrations of BAP alone (2.22, 4.44, or 8.87 μM) or BAP combined with NAA (1.34 or 2.69 μM). The highest mean number of shoots (5.67 shoots per explant) was obtained when the medium contained 8.87 μM BAP and 2.69 μM NAA. The regenerated shoots were readily rooted on MS medium containing 1.0, 2.5, or 5 μM indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA). Rooted regenerants were then transferred to the pots, where they grew well and attained maturity. Over 90% of the regenerants survived the hardening process. The protocol described could be useful for germplasm conservation, commercial cultivation, genetic improvement, and cardenolide production studies in D. lamarckii.

Tercüme edilen katkı başlığıEndemik tıbbi tür Digitalis lamarckii Ivan.'ın kotiledon yapraǧı segmentlerinden indirekt somatik embriyo ve sürgün oluşumu
Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)743-750
Sayfa sayısı8
DergiTurkish Journal of Biology
Hacim35
Basın numarası6
DOI'lar
Yayın durumuYayınlandı - 2011
Harici olarak yayınlandıEvet

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