@inproceedings{04f1900d0ef44414b06521a316821339,
title = "Improving multiphoton microscopy using annular beam shaping, focusing on imaging of human skin",
abstract = "Multiphoton fluorescence microscopy (MPM) is a method for high resolution, non-invasive investigations of biological tissue. The aim of introducing an annular shaped laser beam is to reduce the ouf-of-focus generated background signal improving imaging of light scattering tissue such as human skin. Simulations show that 50% of the beam radius can be blocked, while preserving the shape of the point spread function. Initial experiments performed on a phantom consisting of fluorescein and fluorescent beads embedded in agar by using a custom built MPM-set up show that by introducing a simple beam blocker to create an annular beam, the background signal is reduced with approximately 5%. Future work will include optimizing the set up, and creating phantoms with more light scattering properties.",
keywords = "annular beams, Bessel beams, Multiphoton miocroscopy, two photon microscopy",
author = "Johan Borglin and Durr, {Nicholas J.} and Stina Guldbrand and Onur Ferhanoglu and Wennberg, {Ann Marie} and Dag Hanstorp and Adela Ben-Yakar and Ericson, {Marica B.}",
year = "2014",
doi = "10.1117/12.2038942",
language = "English",
isbn = "9780819498618",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
booktitle = "Multiphoton Microscopy in the Biomedical Sciences XIV",
address = "United States",
note = "Multiphoton Microscopy in the Biomedical Sciences XIV ; Conference date: 02-02-2014 Through 04-02-2014",
}