Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers

S. Tirkeş, L. Toppare*, S. Alkan, U. Bakir, A. Önen, Y. Yǎci

*Bu çalışma için yazışmadan sorumlu yazar

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34 Atıf (Scopus)

Özet

Glucose oxidase (GOD) was immobilized in four different conducting polymer matrices, namely: polypyrrole, (PPy), poly(pyrrole-graft-polytetrahydrofuran), (1) and (3); and poly(pyrrole-graft-polystyrene/polytetrahydrofuran), (2). The kinetic parameters Vmax and Km, and the optimum temperature were determined for both immobilized and native enzymes. The effect of electrolysis time and several supporting electrolytes, p-toluenesulfonic acid, p-toluene sulfonic acid (PTSA), sodium p-toluene sulfonate, sodium p-toluene sulfonate (NaPTS), and sodium dodecyl sulfate, sodium dodecyl sulfate (SDS), on enzyme immobilization were investigated. The high Km value (59.9 mM) of enzyme immobilized in PPy was decreased via immobilization in graft copolymer matrices of pyrrole. Vmax, which was 2.25 mM/min for pure PPy, was found as 4.71 mM/min for compound (3).

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)81-87
Sayfa sayısı7
DergiInternational Journal of Biological Macromolecules
Hacim30
Basın numarası2
DOI'lar
Yayın durumuYayınlandı - 8 Nis 2002

Finansman

This study was supported by State Planning Organization of Turkey (contract no: 98 K 122750) and TUBA.

FinansörlerFinansör numarası
State Planning Organization of Turkey98 K 122750
TUBA

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