Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP)

Gulay Bayramoglu*, B. Filiz Senkal, Meltem Yilmaz, M. Yakup Arica

*Bu çalışma için yazışmadan sorumlu yazar

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38 Atıf (Scopus)

Özet

Poly(hydroxyethyl methacrylate-ethylen glycol dimethacrylate), p(HEMA-EGDMA), beads were prepared by suspension polymerization, and were decorated with fibrous poly(glycidyl methacrylate), p(GMA), via surface initiated-atom transfer radical polymerization (SI-ATRP). The functional epoxy groups of the beads were used for covalent immobilization of papain. The average amount of immobilized enzyme was 18.7mg/g beads. The immobilized enzyme was characterized by temperature, pH, operational and storage stability experiments. The maximum velocity of the free and immobilized enzymes (V max) and Michaelis-Menten constant (K m) values were determined as 10.7 and 8.3U/mg proteins and 274 and 465μM, respectively. The immobilized papain was operated in a batch reactor, and it was very effective for hydrolysis of different proteins (i.e., casein and cytochrom c).

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)9833-9837
Sayfa sayısı5
DergiBioresource Technology
Hacim102
Basın numarası21
DOI'lar
Yayın durumuYayınlandı - Kas 2011

Parmak izi

Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP)' araştırma başlıklarına git. Birlikte benzersiz bir parmak izi oluştururlar.

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