Hot topic: Geographical distribution and strain diversity of Lactobacillus wasatchensis isolated from cheese with unwanted gas formation

Michele Culumber*, Donald J. McMahon, Fatih Ortakci, Lauren Montierth, Beatriz Villalba, Jeffery R. Broadbent, Craig J. Oberg

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6 Atıf (Scopus)

Özet

Lactobacillus wasatchensis, an obligate heterofermentative nonstarter lactic acid bacteria (NSLAB) implicated in causing gas defects in aged cheeses, was originally isolated from an aged Cheddar produced in Logan, Utah. To determine the geographical distribution of this organism, we isolated slow-growing NSLAB from cheeses collected in different regions of the United States, Australia, New Zealand, and Ireland. Seven of the cheeses showed significant gas defects and 12 did not. Nonstarter lactic acid bacteria were isolated from these cheeses on de Man, Rogosa, and Sharpe medium supplemented with ribose, a preferred substrate for Lb. wasatchensis. Identification was confirmed with 16S rRNA gene sequencing and the API50CH (bioMérieux, Marcy l'Etoile, France) carbohydrate panel. Isolates were also compared with one another by using repetitive element sequence-based PCR (rep-PCR). Lactobacillus wasatchensis was isolated only from cheeses demonstrating late-gas development and was found in samples from 6 of the 7 cheeses. This supports laboratory evidence that this organism is a causative agent of late gas production defects. The rep-PCR analysis produced distinct genetic fingerprints for isolates from each cheese, indicating that Lb. wasatchensis is found in several regions across the United States and is not a local phenomenon.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)8764-8767
Sayfa sayısı4
DergiJournal of Dairy Science
Hacim100
Basın numarası11
DOI'lar
Yayın durumuYayınlandı - Kas 2017
Harici olarak yayınlandıEvet

Bibliyografik not

Publisher Copyright:
© 2017 American Dairy Science Association

Finansman

This work has been supported by the Weber State University Department of Microbiology and through the Build Dairy Program at the Western Dairy Center, Logan, Utah.

FinansörlerFinansör numarası
Weber State University Department of Microbiology

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