Green-synthesized silver nanoparticles doped into p(AEMA-co-HEMA) via cryo-polymerization for electrochemical hydrogen peroxide nanocomposite biosensor

The production of highly sensitive electrochemical hydrogen peroxide sensors was essential for detecting the reactivity of hydrogen peroxide in biological systems. Novel, non-enzymatic hydrogen peroxide sensors were developed to offer ultra-high sensitivity and exceptional stability. In this study, a nanocomposite biosensor for hydrogen peroxide detection was designed by doping silver nanoparticles synthesized through green methods into a copolymer matrix of 2-Aminoethylmethacrylamide hydrochloride and 2-Hydroxyethyl methacrylate using a cryogenic polymerization technique. These silver nanoparticles demonstrated antimicrobial properties and enhanced the electrocatalytic activity of the sensor due to their high active surface area. Pistachio hulls and hibiscus leaves were selected as green sources for producing silver nanoparticles, and their effects on electrocatalytic performance were compared. The nanocomposites were characterized morphologically, structurally, and electrochemically. Silver nanoparticles improved detection range and sensitivity even at low concentrations. The electrochemical analyses revealed detection limits of 1.4 micromoles and 3.9 micromoles for sensors using pistachio hulls and hibiscus leaves, respectively. The wide dynamic range (5 pmol to 10 mmol) and low detection limits highlight the potential of these sensors for sensitive, non-enzymatic hydrogen peroxide detection in clinical applications.