Exploring the role of miRNAs in the diagnosis of MODY3

Oğuzhan Fatih Baltaci, Şeyma Çolakoğlu, Gökçe Güllü Amuran, Neslihan Aydin, Mehmet Sarğin, Arzu Karabay, Temel Yilmaz, Ergül Berber*

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6 Atıf (Scopus)

Özet

Background/aim: MODY3 associated with HNF1A is the most common form of MODY and is clinically misdiagnosed as type 1 diabetes due to similar clinical symptoms. This study aimed to analyze the role of HNF1A-regulated miRNAs as a biomarker in the diagnosis of MODY3. Materials and methods: MIN6 cells were transfected with the HNF1A cDNA expression vector for overexpression or with siRNA specific to HNF1A to silence its expression. The HNF1A-regulated miRNAs were determined by RNA-Seq of the total RNA extract. Expressions of the candidate miRNAs in blood samples of MODY3, type 1 diabetes, and type 2 diabetes patients and in healthy subjects were compared statistically by Mann–Whitney U tests. Results: This study revealed the presence of 238 known HNF1A-regulated miRNAs in MIN6 cells. miR-129-1-3p, miR-200b-3p, and miR-378a-5p were selected as candidate miRNAs. The expression level of miR-378a-5p significantly decreased in type 2 diabetes and MODY3 patients, while miR-200b-3p expression was significantly decreased only in MODY3 patients. Conclusion: Although further studies with larger numbers of patients are required, this study demonstrated that the expression levels of miR-200b-3p and miR-378a-5p decrease in MODY3 patients and suggests that miR-200b-3p is an especially strong candidate to use clinically for selecting suspected MODY3 patients.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)620-626
Sayfa sayısı7
DergiTurkish Journal of Medical Sciences
Hacim48
Basın numarası3
DOI'lar
Yayın durumuYayınlandı - 2018

Bibliyografik not

Publisher Copyright:
© TÜBİTAK.

Finansman

This study was supported by the Scientific and Technological Research Council of Turkey (TÜBİTAK Grant Number: 113S217).

FinansörlerFinansör numarası
TÜBİTAK
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu113S217

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