Existence of SARS-CoV-2 RNA on ambient particulate matter samples: A nationwide study in Turkey

Özgecan Kayalar; Akif Arı; Gizem Babuççu; Nur Konyalılar; Özlem Doğan; Füsun Can; Ülkü A. Şahin; Eftade O. Gaga; S. Levent Kuzu; Pelin Ertürk Arı; Mustafa Odabaşı; Yücel Taşdemir; S. Sıddık Cindoruk; Fatma Esen; Egemen Sakın; Burak Çalışkan; Lokman H. Tecer; Merve Fıçıcı; Ahmet Altın; Burcu Onat; Coşkun Ayvaz; Burcu Uzun; Arslan Saral; Tuncay Döğeroğlu; Semra Malkoç; Özlem Özden Üzmez; Fatma Kunt; Senar Aydın; Melik Kara; Barış Yaman; Güray Doğan; Bihter Olgun; Ebru N. Dokumacı; Gülen Güllü; Elif S. Uzunpınar; Hasan Bayram

doi:10.1016/j.scitotenv.2021.147976

Existence of SARS-CoV-2 RNA on ambient particulate matter samples: A nationwide study in Turkey

Özgecan Kayalar
, Akif Arı
, Gizem Babuççu
, Nur Konyalılar
, Özlem Doğan
, Füsun Can
, Ülkü A. Şahin
, Eftade O. Gaga
, S. Levent Kuzu
, Pelin Ertürk Arı
, Mustafa Odabaşı
, Yücel Taşdemir
, S. Sıddık Cindoruk
, Fatma Esen
, Egemen Sakın
, Burak Çalışkan
, Lokman H. Tecer
, Merve Fıçıcı
, Ahmet Altın
, Burcu Onat

Coşkun Ayvaz, Burcu Uzun, Arslan Saral, Tuncay Döğeroğlu, Semra Malkoç, Özlem Özden Üzmez, Fatma Kunt, Senar Aydın, Melik Kara, Barış Yaman, Güray Doğan, Bihter Olgun, Ebru N. Dokumacı, Gülen Güllü, Elif S. Uzunpınar, Hasan Bayram^*

^*Bu çalışma için yazışmadan sorumlu yazar

Koc University
Abant Izzet Baysal University
Istanbul University - Cerrahpaşa
Eskisehir Technical University
Yildiz Technical University
Dokuz Eylul University
Uludag University
Namik Kemal University
Zonguldak Bülent Ecevit University
Necmettin Erbakan University
Akdeniz University
Hacettepe University
Middle East Technical University

Araştırma sonucu: Dergiye katkı › Makale › bilirkişi

43 Atıf (Scopus)

Özet

Coronavirus disease 2019 (COVID-19) is caused by the SARS-CoV-2 virus and has been affecting the world since the end of 2019. The disease led to significant mortality and morbidity in Turkey, since the first case was reported on March 11th, 2020. Studies suggest a positive association between air pollution and SARS-CoV-2 infection. The aim of the present study was to investigate the role of ambient particulate matters (PM), as potential carriers for SARS-CoV-2. Ambient PM samples in various size ranges were collected from 13 sites including urban and urban-background locations and hospital gardens in 10 cities across Turkey between 13th of May and 14th of June 2020 to investigate the possible presence of SARS-CoV-2 RNA on ambient PM. A total of 203 daily samples (TSP, n = 80; PM_2.5, n = 33; PM_2.5_–₁₀, n = 23; PM₁₀μm, n = 19; and 6 size segregated PM, n = 48) were collected using various samplers. The N1 gene and RdRP gene expressions were analyzed for the presence of SARS-CoV-2, as suggested by the Centers for Disease Control and Prevention (CDC). According to real time (RT)-PCR and three-dimensional (3D) digital (d) PCR analysis, dual RdRP and N1 gene positivity were detected in 20 (9.8%) samples. Ambient PM-bound SARS-CoV-2 was analyzed quantitatively and the air concentrations of the virus ranged from 0.1 copies/m³ to 23 copies/m³. The highest percentages of virus detection on PM samples were from hospital gardens in Tekirdağ, Zonguldak, and Istanbul, especially in PM_2.5 mode. Findings of this study have suggested that SARS-CoV-2 may be transported by ambient particles, especially at sites close to the infection hot-spots. However, whether this has an impact on the spread of the virus infection remains to be determined.

Orijinal dil	İngilizce
Makale numarası	147976
Dergi	Science of the Total Environment
Hacim	789
DOI'lar	https://doi.org/10.1016/j.scitotenv.2021.147976
Yayın durumu	Yayınlandı - 1 Eki 2021
Harici olarak yayınlandı	Evet

Bibliyografik not

Publisher Copyright:
© 2021 Elsevier B.V.

BM SKH

Bu sonuç, aşağıdaki Sürdürülebilir Kalkınma Hedefine/Hedeflerine katkıda bulunur

SKH 3 Sağlık ve Kaliteli Yaşam
SKH 11 Sürdürülebilir Şehirler ve Topluluklar
SKH 14 Sudaki Yaşam

Belgeye Erişim

10.1016/j.scitotenv.2021.147976

Diğer Dosyalar ve Linkler

Link to publication in Scopus

Alıntı Yap

Kayalar, Ö., Arı, A., Babuççu, G., Konyalılar, N., Doğan, Ö., Can, F., Şahin, Ü. A., Gaga, E. O., Levent Kuzu, S., Arı, P. E., Odabaşı, M., Taşdemir, Y., Sıddık Cindoruk, S., Esen, F., Sakın, E., Çalışkan, B., Tecer, L. H., Fıçıcı, M., Altın, A., ... Bayram, H. (2021). Existence of SARS-CoV-2 RNA on ambient particulate matter samples: A nationwide study in Turkey. Science of the Total Environment, 789, Makale 147976. https://doi.org/10.1016/j.scitotenv.2021.147976

@article{6e0da06860c94b338426867a1eefdce1,

title = "Existence of SARS-CoV-2 RNA on ambient particulate matter samples: A nationwide study in Turkey",

abstract = "Coronavirus disease 2019 (COVID-19) is caused by the SARS-CoV-2 virus and has been affecting the world since the end of 2019. The disease led to significant mortality and morbidity in Turkey, since the first case was reported on March 11th, 2020. Studies suggest a positive association between air pollution and SARS-CoV-2 infection. The aim of the present study was to investigate the role of ambient particulate matters (PM), as potential carriers for SARS-CoV-2. Ambient PM samples in various size ranges were collected from 13 sites including urban and urban-background locations and hospital gardens in 10 cities across Turkey between 13th of May and 14th of June 2020 to investigate the possible presence of SARS-CoV-2 RNA on ambient PM. A total of 203 daily samples (TSP, n = 80; PM2.5, n = 33; PM2.5–10, n = 23; PM10μm, n = 19; and 6 size segregated PM, n = 48) were collected using various samplers. The N1 gene and RdRP gene expressions were analyzed for the presence of SARS-CoV-2, as suggested by the Centers for Disease Control and Prevention (CDC). According to real time (RT)-PCR and three-dimensional (3D) digital (d) PCR analysis, dual RdRP and N1 gene positivity were detected in 20 (9.8\%) samples. Ambient PM-bound SARS-CoV-2 was analyzed quantitatively and the air concentrations of the virus ranged from 0.1 copies/m3 to 23 copies/m3. The highest percentages of virus detection on PM samples were from hospital gardens in Tekirdağ, Zonguldak, and Istanbul, especially in PM2.5 mode. Findings of this study have suggested that SARS-CoV-2 may be transported by ambient particles, especially at sites close to the infection hot-spots. However, whether this has an impact on the spread of the virus infection remains to be determined.",

keywords = "3D-dPCR, Airborne, Particulate matter, SARS-CoV-2",

author = "{\"O}zgecan Kayalar and Akif Arı and Gizem Babu{\c c}{\c c}u and Nur Konyalılar and {\"O}zlem Doğan and F{\"u}sun Can and {\c S}ahin, \{{\"U}lk{\"u} A.\} and Gaga, \{Eftade O.\} and \{Levent Kuzu\}, S. and Arı, \{Pelin Ert{\"u}rk\} and Mustafa Odaba{\c s}ı and Y{\"u}cel Ta{\c s}demir and \{Sıddık Cindoruk\}, S. and Fatma Esen and Egemen Sakın and Burak {\c C}alı{\c s}kan and Tecer, \{Lokman H.\} and Merve Fı{\c c}ıcı and Ahmet Altın and Burcu Onat and Co{\c s}kun Ayvaz and Burcu Uzun and Arslan Saral and Tuncay D{\"o}ğeroğlu and Semra Malko{\c c} and {\"U}zmez, \{{\"O}zlem {\"O}zden\} and Fatma Kunt and Senar Aydın and Melik Kara and Barı{\c s} Yaman and G{\"u}ray Doğan and Bihter Olgun and Dokumacı, \{Ebru N.\} and G{\"u}len G{\"u}ll{\"u} and Uzunpınar, \{Elif S.\} and Hasan Bayram",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.",

year = "2021",

month = oct,

day = "1",

doi = "10.1016/j.scitotenv.2021.147976",

language = "English",

volume = "789",

journal = "Science of the Total Environment",

issn = "0048-9697",

publisher = "Elsevier B.V.",

}

Kayalar, Ö, Arı, A, Babuççu, G, Konyalılar, N, Doğan, Ö, Can, F, Şahin, ÜA, Gaga, EO, Levent Kuzu, S, Arı, PE, Odabaşı, M, Taşdemir, Y, Sıddık Cindoruk, S, Esen, F, Sakın, E, Çalışkan, B, Tecer, LH, Fıçıcı, M, Altın, A, Onat, B, Ayvaz, C, Uzun, B, Saral, A, Döğeroğlu, T, Malkoç, S, Üzmez, ÖÖ, Kunt, F, Aydın, S, Kara, M, Yaman, B, Doğan, G, Olgun, B, Dokumacı, EN, Güllü, G, Uzunpınar, ES & Bayram, H 2021, 'Existence of SARS-CoV-2 RNA on ambient particulate matter samples: A nationwide study in Turkey', Science of the Total Environment, hac. 789, 147976. https://doi.org/10.1016/j.scitotenv.2021.147976

TY - JOUR

T1 - Existence of SARS-CoV-2 RNA on ambient particulate matter samples

T2 - A nationwide study in Turkey

AU - Kayalar, Özgecan

AU - Arı, Akif

AU - Babuççu, Gizem

AU - Konyalılar, Nur

AU - Doğan, Özlem

AU - Can, Füsun

AU - Şahin, Ülkü A.

AU - Gaga, Eftade O.

AU - Levent Kuzu, S.

AU - Arı, Pelin Ertürk

AU - Odabaşı, Mustafa

AU - Taşdemir, Yücel

AU - Sıddık Cindoruk, S.

AU - Esen, Fatma

AU - Sakın, Egemen

AU - Çalışkan, Burak

AU - Tecer, Lokman H.

AU - Fıçıcı, Merve

AU - Altın, Ahmet

AU - Onat, Burcu

AU - Ayvaz, Coşkun

AU - Uzun, Burcu

AU - Saral, Arslan

AU - Döğeroğlu, Tuncay

AU - Malkoç, Semra

AU - Üzmez, Özlem Özden

AU - Kunt, Fatma

AU - Aydın, Senar

AU - Kara, Melik

AU - Yaman, Barış

AU - Doğan, Güray

AU - Olgun, Bihter

AU - Dokumacı, Ebru N.

AU - Güllü, Gülen

AU - Uzunpınar, Elif S.

AU - Bayram, Hasan

PY - 2021/10/1

Y1 - 2021/10/1

N2 - Coronavirus disease 2019 (COVID-19) is caused by the SARS-CoV-2 virus and has been affecting the world since the end of 2019. The disease led to significant mortality and morbidity in Turkey, since the first case was reported on March 11th, 2020. Studies suggest a positive association between air pollution and SARS-CoV-2 infection. The aim of the present study was to investigate the role of ambient particulate matters (PM), as potential carriers for SARS-CoV-2. Ambient PM samples in various size ranges were collected from 13 sites including urban and urban-background locations and hospital gardens in 10 cities across Turkey between 13th of May and 14th of June 2020 to investigate the possible presence of SARS-CoV-2 RNA on ambient PM. A total of 203 daily samples (TSP, n = 80; PM2.5, n = 33; PM2.5–10, n = 23; PM10μm, n = 19; and 6 size segregated PM, n = 48) were collected using various samplers. The N1 gene and RdRP gene expressions were analyzed for the presence of SARS-CoV-2, as suggested by the Centers for Disease Control and Prevention (CDC). According to real time (RT)-PCR and three-dimensional (3D) digital (d) PCR analysis, dual RdRP and N1 gene positivity were detected in 20 (9.8%) samples. Ambient PM-bound SARS-CoV-2 was analyzed quantitatively and the air concentrations of the virus ranged from 0.1 copies/m3 to 23 copies/m3. The highest percentages of virus detection on PM samples were from hospital gardens in Tekirdağ, Zonguldak, and Istanbul, especially in PM2.5 mode. Findings of this study have suggested that SARS-CoV-2 may be transported by ambient particles, especially at sites close to the infection hot-spots. However, whether this has an impact on the spread of the virus infection remains to be determined.

AB - Coronavirus disease 2019 (COVID-19) is caused by the SARS-CoV-2 virus and has been affecting the world since the end of 2019. The disease led to significant mortality and morbidity in Turkey, since the first case was reported on March 11th, 2020. Studies suggest a positive association between air pollution and SARS-CoV-2 infection. The aim of the present study was to investigate the role of ambient particulate matters (PM), as potential carriers for SARS-CoV-2. Ambient PM samples in various size ranges were collected from 13 sites including urban and urban-background locations and hospital gardens in 10 cities across Turkey between 13th of May and 14th of June 2020 to investigate the possible presence of SARS-CoV-2 RNA on ambient PM. A total of 203 daily samples (TSP, n = 80; PM2.5, n = 33; PM2.5–10, n = 23; PM10μm, n = 19; and 6 size segregated PM, n = 48) were collected using various samplers. The N1 gene and RdRP gene expressions were analyzed for the presence of SARS-CoV-2, as suggested by the Centers for Disease Control and Prevention (CDC). According to real time (RT)-PCR and three-dimensional (3D) digital (d) PCR analysis, dual RdRP and N1 gene positivity were detected in 20 (9.8%) samples. Ambient PM-bound SARS-CoV-2 was analyzed quantitatively and the air concentrations of the virus ranged from 0.1 copies/m3 to 23 copies/m3. The highest percentages of virus detection on PM samples were from hospital gardens in Tekirdağ, Zonguldak, and Istanbul, especially in PM2.5 mode. Findings of this study have suggested that SARS-CoV-2 may be transported by ambient particles, especially at sites close to the infection hot-spots. However, whether this has an impact on the spread of the virus infection remains to be determined.

KW - 3D-dPCR

KW - Airborne

KW - Particulate matter

KW - SARS-CoV-2

UR - https://www.scopus.com/pages/publications/85107044697

U2 - 10.1016/j.scitotenv.2021.147976

DO - 10.1016/j.scitotenv.2021.147976

M3 - Article

C2 - 34058581

AN - SCOPUS:85107044697

SN - 0048-9697

VL - 789

JO - Science of the Total Environment

JF - Science of the Total Environment

M1 - 147976

ER -

Existence of SARS-CoV-2 RNA on ambient particulate matter samples: A nationwide study in Turkey

Özet

Bibliyografik not

BM SKH

Belgeye Erişim

Diğer Dosyalar ve Linkler

Parmak izi

Alıntı Yap