TY - JOUR
T1 - Evolutionary engineering and transcriptomic analysis of nickel-resistant Saccharomyces cerevisiae
AU - Küçükgöze, Gökhan
AU - Alkim, Ceren
AU - Yilmaz, Ülkü
AU - Kisakesen, H. Ibrahim
AU - Gündüz, Sema
AU - Akman, Süleyman
AU - Çakar, Z. Petek
PY - 2013/12
Y1 - 2013/12
N2 - Increased exposure to nickel compounds and alloys due to industrial development has resulted in nickel pollution and many pathological effects on human health. However, there is very limited information about nickel response, transport, and tolerance in eukaryotes. To investigate nickel resistance in the model eukaryote Saccharomyces cerevisiae, evolutionary engineering by batch selection under gradually increasing nickel stress levels was performed. Nickel hyper-resistant mutants that could resist up to 5.3 mM NiCl2, a lethal level for the reference strain, were selected. The mutants were also cross-resistant against iron, cobalt, zinc, and manganese stresses and accumulated more than twofold higher nickel than the reference strain. Global transcriptomic analysis revealed that 640 upregulated genes were related to iron homeostasis, stress response, and oxidative damage, implying that nickel resistance may share common mechanisms with iron and cobalt resistance, general stress response, and oxidative damage.
AB - Increased exposure to nickel compounds and alloys due to industrial development has resulted in nickel pollution and many pathological effects on human health. However, there is very limited information about nickel response, transport, and tolerance in eukaryotes. To investigate nickel resistance in the model eukaryote Saccharomyces cerevisiae, evolutionary engineering by batch selection under gradually increasing nickel stress levels was performed. Nickel hyper-resistant mutants that could resist up to 5.3 mM NiCl2, a lethal level for the reference strain, were selected. The mutants were also cross-resistant against iron, cobalt, zinc, and manganese stresses and accumulated more than twofold higher nickel than the reference strain. Global transcriptomic analysis revealed that 640 upregulated genes were related to iron homeostasis, stress response, and oxidative damage, implying that nickel resistance may share common mechanisms with iron and cobalt resistance, general stress response, and oxidative damage.
KW - Evolutionary engineering
KW - Inverse metabolic engineering
KW - Nickel resistance
KW - Saccharomyces cerevisiae
KW - Stress resistance
KW - Yeast DNA microarray analysis
UR - http://www.scopus.com/inward/record.url?scp=84887608790&partnerID=8YFLogxK
U2 - 10.1111/1567-1364.12073
DO - 10.1111/1567-1364.12073
M3 - Article
C2 - 23992612
AN - SCOPUS:84887608790
SN - 1567-1356
VL - 13
SP - 731
EP - 746
JO - FEMS Yeast Research
JF - FEMS Yeast Research
IS - 8
ER -
