Enhanced osteoclast like-cell functions on nanophase ceramics

Thomas J. Webster; Celaletdin Ergun; Robert H. Doremus; Richard W. Siegel; Rena Bizios

Enhanced osteoclast like-cell functions on nanophase ceramics

Thomas J. Webster, Celaletdin Ergun, Robert H. Doremus, Richard W. Siegel, Rena Bizios

Araştırma sonucu: Dergiye katkı › Konferans makalesi › bilirkişi

1 Atıf (Scopus)

Özet

The present in vitro study investigated osteoclast-like cell (the boneresorbing cells) functions on nanophase ceramics. For this purpose, rat bone-marrow cells were seeded onto nanophase and conventional alumina and hydroxyapatite substrates and cultured under standard cell culture conditions for 7, 10, and 13 days. After the prescribed time period, synthesis of tartrate-resistant acid phosphatase (TRAP) was determined in the lysates of cells using a commercially available kit (Sigma). Resorption pits on the ceramic surfaces were visualized and counted using reflected light microscopy (Olympus I×70) with image analysis software (Image Pro).

Orijinal dil	İngilizce
Sayfa (başlangıç-bitiş)	S-15
Dergi	Annals of Biomedical Engineering
Hacim	28
Basın numarası	SUPPL. 1
Yayın durumu	Yayınlandı - 2000
Harici olarak yayınlandı	Evet
Etkinlik	2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA Süre: 12 Eki 2000 → 14 Eki 2000

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@article{efd7f967acfe423991433ab42b312d58,

title = "Enhanced osteoclast like-cell functions on nanophase ceramics",

abstract = "The present in vitro study investigated osteoclast-like cell (the boneresorbing cells) functions on nanophase ceramics. For this purpose, rat bone-marrow cells were seeded onto nanophase and conventional alumina and hydroxyapatite substrates and cultured under standard cell culture conditions for 7, 10, and 13 days. After the prescribed time period, synthesis of tartrate-resistant acid phosphatase (TRAP) was determined in the lysates of cells using a commercially available kit (Sigma). Resorption pits on the ceramic surfaces were visualized and counted using reflected light microscopy (Olympus I×70) with image analysis software (Image Pro).",

author = "Webster, {Thomas J.} and Celaletdin Ergun and Doremus, {Robert H.} and Siegel, {Richard W.} and Rena Bizios",

year = "2000",

language = "English",

volume = "28",

pages = "S--15",

journal = "Annals of Biomedical Engineering",

issn = "0090-6964",

publisher = "Springer",

number = "SUPPL. 1",

note = "2000 Annual Fall Meeting of the Biomedical Engineering Society ; Conference date: 12-10-2000 Through 14-10-2000",

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TY - JOUR

T1 - Enhanced osteoclast like-cell functions on nanophase ceramics

AU - Webster, Thomas J.

AU - Ergun, Celaletdin

AU - Doremus, Robert H.

AU - Siegel, Richard W.

AU - Bizios, Rena

PY - 2000

Y1 - 2000

N2 - The present in vitro study investigated osteoclast-like cell (the boneresorbing cells) functions on nanophase ceramics. For this purpose, rat bone-marrow cells were seeded onto nanophase and conventional alumina and hydroxyapatite substrates and cultured under standard cell culture conditions for 7, 10, and 13 days. After the prescribed time period, synthesis of tartrate-resistant acid phosphatase (TRAP) was determined in the lysates of cells using a commercially available kit (Sigma). Resorption pits on the ceramic surfaces were visualized and counted using reflected light microscopy (Olympus I×70) with image analysis software (Image Pro).

AB - The present in vitro study investigated osteoclast-like cell (the boneresorbing cells) functions on nanophase ceramics. For this purpose, rat bone-marrow cells were seeded onto nanophase and conventional alumina and hydroxyapatite substrates and cultured under standard cell culture conditions for 7, 10, and 13 days. After the prescribed time period, synthesis of tartrate-resistant acid phosphatase (TRAP) was determined in the lysates of cells using a commercially available kit (Sigma). Resorption pits on the ceramic surfaces were visualized and counted using reflected light microscopy (Olympus I×70) with image analysis software (Image Pro).

UR - http://www.scopus.com/inward/record.url?scp=0034508284&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:0034508284

SN - 0090-6964

VL - 28

SP - S-15

JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

IS - SUPPL. 1

T2 - 2000 Annual Fall Meeting of the Biomedical Engineering Society

Y2 - 12 October 2000 through 14 October 2000

ER -

Enhanced osteoclast like-cell functions on nanophase ceramics

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