Embryo viability indexing using Raman spectroscopy of spent culture media

Uğur Parlatan; Günay Başar; Nima Bavili; Harika Yumru; Fatih Sag; Sibel Bulgurcuoglu; Ercan Bastu

doi:10.1080/00387010.2016.1198814

Embryo viability indexing using Raman spectroscopy of spent culture media

Uğur Parlatan^*
, Günay Başar
, Nima Bavili
, Harika Yumru
, Fatih Sag
, Sibel Bulgurcuoglu
, Ercan Bastu

^*Bu çalışma için yazışmadan sorumlu yazar

Fizik Mühendisliği Bölümü

Istanbul Technical University
Istanbul University

Araştırma sonucu: Dergiye katkı › Makale › bilirkişi

5 Atıf (Scopus)

Özet

Embryo viability quantification is an important topic for in vitro fertilization researchers. This study suggests Raman spectroscopy as a candidate method to find an objective measure of embryo viability. Raman spectra of 31 spent embryo culture samples (16 nonpregnant, 15 pregnant) from 31 patients were analyzed with band component analysis. The band area ratio of 902 to 943 cm^-1 was found to be the most discriminatory. These bands are related to glutamine, glycine, proline, aspartic acid, and valine. According to the pregnancy outcome the sensitivity and specificity of Raman analysis were found to be 93% and 77%, respectively.

Orijinal dil	İngilizce
Sayfa (başlangıç-bitiş)	458-463
Sayfa sayısı	6
Dergi	Spectroscopy Letters
Hacim	49
Basın numarası	7
DOI'lar	https://doi.org/10.1080/00387010.2016.1198814
Yayın durumu	Yayınlandı - 17 Haz 2016

Bibliyografik not

Publisher Copyright:
© 2016 Taylor & Francis.

Belgeye Erişim

10.1080/00387010.2016.1198814

Diğer Dosyalar ve Linkler

Link to publication in Scopus

Alıntı Yap

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title = "Embryo viability indexing using Raman spectroscopy of spent culture media",

abstract = "Embryo viability quantification is an important topic for in vitro fertilization researchers. This study suggests Raman spectroscopy as a candidate method to find an objective measure of embryo viability. Raman spectra of 31 spent embryo culture samples (16 nonpregnant, 15 pregnant) from 31 patients were analyzed with band component analysis. The band area ratio of 902 to 943 cm-1 was found to be the most discriminatory. These bands are related to glutamine, glycine, proline, aspartic acid, and valine. According to the pregnancy outcome the sensitivity and specificity of Raman analysis were found to be 93\% and 77\%, respectively.",

keywords = "Band component analysis, Embryo viability, In vitro fertilization, Raman spectroscopy",

author = "Uğur Parlatan and G{\"u}nay Ba{\c s}ar and Nima Bavili and Harika Yumru and Fatih Sag and Sibel Bulgurcuoglu and Ercan Bastu",

note = "Publisher Copyright: {\textcopyright} 2016 Taylor \& Francis.",

year = "2016",

month = jun,

day = "17",

doi = "10.1080/00387010.2016.1198814",

language = "English",

volume = "49",

pages = "458--463",

journal = "Spectroscopy Letters",

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}

TY - JOUR

T1 - Embryo viability indexing using Raman spectroscopy of spent culture media

AU - Parlatan, Uğur

AU - Başar, Günay

AU - Bavili, Nima

AU - Yumru, Harika

AU - Sag, Fatih

AU - Bulgurcuoglu, Sibel

AU - Bastu, Ercan

PY - 2016/6/17

Y1 - 2016/6/17

N2 - Embryo viability quantification is an important topic for in vitro fertilization researchers. This study suggests Raman spectroscopy as a candidate method to find an objective measure of embryo viability. Raman spectra of 31 spent embryo culture samples (16 nonpregnant, 15 pregnant) from 31 patients were analyzed with band component analysis. The band area ratio of 902 to 943 cm-1 was found to be the most discriminatory. These bands are related to glutamine, glycine, proline, aspartic acid, and valine. According to the pregnancy outcome the sensitivity and specificity of Raman analysis were found to be 93% and 77%, respectively.

AB - Embryo viability quantification is an important topic for in vitro fertilization researchers. This study suggests Raman spectroscopy as a candidate method to find an objective measure of embryo viability. Raman spectra of 31 spent embryo culture samples (16 nonpregnant, 15 pregnant) from 31 patients were analyzed with band component analysis. The band area ratio of 902 to 943 cm-1 was found to be the most discriminatory. These bands are related to glutamine, glycine, proline, aspartic acid, and valine. According to the pregnancy outcome the sensitivity and specificity of Raman analysis were found to be 93% and 77%, respectively.

KW - Band component analysis

KW - Embryo viability

KW - In vitro fertilization

KW - Raman spectroscopy

UR - https://www.scopus.com/pages/publications/84983349104

U2 - 10.1080/00387010.2016.1198814

DO - 10.1080/00387010.2016.1198814

M3 - Article

AN - SCOPUS:84983349104

SN - 0038-7010

VL - 49

SP - 458

EP - 463

JO - Spectroscopy Letters

JF - Spectroscopy Letters

IS - 7

ER -

Embryo viability indexing using Raman spectroscopy of spent culture media

Özet

Bibliyografik not

Belgeye Erişim

Diğer Dosyalar ve Linkler

Parmak izi

Alıntı Yap