Determination of urinary succinylacetone by capillary electrophoresis for the diagnosis of tyrosinemia type I

M. Şerif Cansever, F. Bedia Erim*

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: ???type-name???Makalebilirkişi

8 Atıf (Scopus)

Özet

The presence of succinylacetone in urine or blood or amniotic fluid is pathognomonic of an inherited metabolic disorder, named tyrosinemia type I. We developed a capillary electrophoretic method for the fast analysis of succinylacetone in urine samples. The separation was performed at reversed polarity mode using either a cationic surfactant as the buffer additive, or a capillary coated with a positively charged polyelectrolyte. Under these conditions, urine samples were directly injected to the capillary without any pretreatment step. The utility of the method was demonstrated by the identification of succinyacetone in urine from patients with hereditary tyrosinemia type I. For all patients, diagnostic peaks at the expected migration times were detected. The developed method is rapid, simple, inexpensive, and suitable for the determination of succinylacetone in clinical urine samples.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)309-311
Sayfa sayısı3
DergiJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Hacim818
Basın numarası2
DOI'lar
Yayın durumuYayınlandı - 25 Nis 2005

Parmak izi

Determination of urinary succinylacetone by capillary electrophoresis for the diagnosis of tyrosinemia type I' araştırma başlıklarına git. Birlikte benzersiz bir parmak izi oluştururlar.

Alıntı Yap