Contents of selected B vitamins in NIST SRM 3280 multivitamin/multielement tablets by liquid chromatography isotope dilution mass spectrometry

Pei Chen, Mustafa Ozcan, Wayne R. Wolf*

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17 Atıf (Scopus)

Özet

There is increased interest in accurately assessing the total dietary intake of vitamins from all sources, including foods and dietary supplements. Consequently, a Dietary Supplement Ingredient Database (DSID), based upon analytical values, is being established by USDA with support of the Office of Dietary Supplements (ODS), NIH. The DSID necessitated the development of a new SRM, 3280 - Multivitamin/Multimineral Tablets, by the National Institute of Standards and Technology (NIST), with support from the ODS. As a continuation of a long-term project to develop and validate new methods of determining water-soluble B vitamins in foods and dietary supplements, and as part of a collaborative effort with NIST to characterize SRM 3280, values for the vitamin contents of SRM 3280 have been generated by a liquid chromatographic isotope dilution mass spectrometric (LC/IDMS) method. Isotope-labeled (13C and/or 2H) B vitamins (B1-thiamine, B6-pyridoxine, B3-nicotinamide, and B5-pantothenic acid) were obtained from commercial sources, with the support of the ODS/NIH. Our LC/IDMS method uses a C18 reversed phase column, an Agilent 1100 HPLC system, and a Quattro Micro triple-quad mass spectrometer (MS). B vitamin determination was achieved using a gradient LC profile combined with MS/MS detection in multiple reaction monitoring mode. Stock solutions of the isotope-labeled vitamins were calibrated against USP standard solutions. The SRM tablets, with added amounts of the four isotope-labeled B vitamins, were extracted and the vitamins simultaneously determined in a single LC run, in contrast with the single-component determinations performed via IDMS. Unknown vitamin concentrations were calculated by comparing the ratios of the integrated LC peaks at the different masses of the unlabeled and labeled vitamins.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)343-347
Sayfa sayısı5
DergiAnalytical and Bioanalytical Chemistry
Hacim389
Basın numarası1
DOI'lar
Yayın durumuYayınlandı - Eyl 2007
Harici olarak yayınlandıEvet

Finansman

In order to address increased interest in accurately assessing the total dietary intake of vitamins from all sources, including both dietary supplements and foods, a Dietary Supplement Ingredient Database (DSID), comprising analytical values, is under joint development by the Agricultural Research Service (ARS, USDA) and the Office of Dietary Supplements of the National Institutes of Health (ODS/NIH) [1]. Generation of valid analytical data by contract analysis for the DSID necessitates the availability of appropriate control materials and very importantly the availability of an appropriate Standard Reference Material to evaluate the accuracy of the data. Consequently, as a part of these efforts to establish the DSID, contracts were let by ODS/NIH to the National Institute of Standards and Technology (NIST) to produce a new Standard Reference Material: SRM 3280 Multivitamins/Multielement Tablets. Characterization of a SRM requires that information be generated by multiple analytical techniques, ideally including isotope dilution mass spectrometry (IDMS), which can be a definitive analytical method for very accurate concentration determinations [2]. Abstract There is increased interest in accurately assessing the total dietary intake of vitamins from all sources, including foods and dietary supplements. Consequently, a Dietary Supplement Ingredient Database (DSID), based upon analytical values, is being established by USDA with support of the Office of Dietary Supplements (ODS), NIH. The DSID necessitated the development of a new SRM, 3280 — Multivitamin/Multimineral Tablets, by the National Institute of Standards and Technology (NIST), with support from the ODS. As a continuation of a long-term project to develop and validate new methods of determining water-soluble B vitamins in foods and dietary supplements, and as part of a collaborative effort with NIST to characterize SRM 3280, values for the vitamin contents of SRM 3280 have been generated by a liquid chromatographic isotope dilution mass spectrometric (LC/IDMS) method. Isotope-labeled (13C and/or 2H) B vitamins (B1-thiamine, B6-pyridoxine, B3-nicotinamide, and B5-pantothenic acid) were obtained from commercial sources, with the support of the ODS/NIH. Our LC/IDMS method uses a C18 reversed phase column, an Agilent 1100 HPLC system, and a Quattro Micro triple-quad mass spectrometer (MS). B vitamin determination was achieved using a gradient LC profile combined with MS/MS detection in multiple reaction monitoring mode. Stock solutions of the isotope-labeled vitamins were calibrated against USP standard solutions. The SRM tablets, with added amounts of the four isotope-labeled B vitamins, were extracted and the

FinansörlerFinansör numarası
National Institutes of Health
Office of Dietary Supplements
National Institute of Standards and TechnologyNIST
U.S. Department of Agriculture
Agricultural Research Service
Total

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