Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production

Abdulmecit Gökçe; Yavuz Öztürk; Zeynep Petek Çakar

doi:10.1016/j.ijhydene.2017.07.005

Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production

Abdulmecit Gökçe, Yavuz Öztürk, Zeynep Petek Çakar^*

^*Bu çalışma için yazışmadan sorumlu yazar

Mol.Biyoloji ve Genetik Bölümü

Araştırma sonucu: ???type-name??? › Makale › bilirkişi

5 Atıf (Scopus)

Özet

To overcome efficiency losses due to overheating in outdoor photobioreactors for microbial hydrogen production, heat-resistant microorganisms are desirable. In this study, the comparative whole genome sequencing analyses of two previously obtained Rhodobacter capsulatus heat-resistant mutants; A52 and B41, with modified hydrogen production capacities, have been performed to identify mutations related to hydrogen production. In comparison with the reference strain DSM1710, the genomes of the mutants A52 and B41 contained 2137 and 2253 mutations, respectively. In the mutant B41 genome, mutations were characterized within the nifD, nifJ, glnD, nifB1, ccpA, hupD, dmsA, and cbbR1 genes. On the other hand; in the A52 mutant, mutations were characterized in the nifB2, rnfF, nifJ, cbbO, anfH, amt, moeA, and hupD genes. The effects of nitrogen metabolism and redox-related mutations were tested by quantitative reverse transcription PCR. The most promising mutation was found on the glnD gene of B41 strain with higher hydrogen production capacity.

Orijinal dil	İngilizce
Sayfa (başlangıç-bitiş)	20529-20539
Sayfa sayısı	11
Dergi	International Journal of Hydrogen Energy
Hacim	42
Basın numarası	32
DOI'lar	https://doi.org/10.1016/j.ijhydene.2017.07.005
Yayın durumu	Yayınlandı - 10 Ağu 2017

Bibliyografik not

Publisher Copyright:
© 2017 Hydrogen Energy Publications LLC

Finansman

This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) [grant number 214Z138 (PI: Z. P. Cakar)]; Istanbul Technical University Research Funds for Ph.D. Thesis Projects [grant number 39572 (PI: Z. P. Cakar)]. Authors express their thanks to Bekir Ergüner, National Research Institute of Electronics and Cryptology, Advanced Genome & Bioinformatics Research Laboratory (TUBITAK), for the sequencing analyses.

Finansörler	Finansör numarası
Advanced Genome & Bioinformatics Research Laboratory
National Research Institute of Electronics and Cryptology
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu	214Z138
Istanbul Teknik Üniversitesi	39572

BM SKH

Bu sonuç, aşağıdaki Sürdürülebilir Kalkınma Hedefine/Hedeflerine katkıda bulunur

Belgeye Erişim

10.1016/j.ijhydene.2017.07.005

Diğer Dosyalar ve Linkler

Link to publication in Scopus

Alıntı Yap

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title = "Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production",

abstract = "To overcome efficiency losses due to overheating in outdoor photobioreactors for microbial hydrogen production, heat-resistant microorganisms are desirable. In this study, the comparative whole genome sequencing analyses of two previously obtained Rhodobacter capsulatus heat-resistant mutants; A52 and B41, with modified hydrogen production capacities, have been performed to identify mutations related to hydrogen production. In comparison with the reference strain DSM1710, the genomes of the mutants A52 and B41 contained 2137 and 2253 mutations, respectively. In the mutant B41 genome, mutations were characterized within the nifD, nifJ, glnD, nifB1, ccpA, hupD, dmsA, and cbbR1 genes. On the other hand; in the A52 mutant, mutations were characterized in the nifB2, rnfF, nifJ, cbbO, anfH, amt, moeA, and hupD genes. The effects of nitrogen metabolism and redox-related mutations were tested by quantitative reverse transcription PCR. The most promising mutation was found on the glnD gene of B41 strain with higher hydrogen production capacity.",

keywords = "Bacterial genomics, Biohydrogen, Next generation sequencing, PII uridylyl-transferase, Purple nonsulfur bacteria",

author = "Abdulmecit G{\"o}k{\c c}e and Yavuz {\"O}zt{\"u}rk and {\c C}akar, {Zeynep Petek}",

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year = "2017",

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doi = "10.1016/j.ijhydene.2017.07.005",

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Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production. / Gökçe, Abdulmecit; Öztürk, Yavuz; Çakar, Zeynep Petek.
In: International Journal of Hydrogen Energy, Hac. 42, No. 32, 10.08.2017, p. 20529-20539.

Araştırma sonucu: ???type-name??? › Makale › bilirkişi

TY - JOUR

T1 - Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production

AU - Gökçe, Abdulmecit

AU - Öztürk, Yavuz

AU - Çakar, Zeynep Petek

PY - 2017/8/10

Y1 - 2017/8/10

N2 - To overcome efficiency losses due to overheating in outdoor photobioreactors for microbial hydrogen production, heat-resistant microorganisms are desirable. In this study, the comparative whole genome sequencing analyses of two previously obtained Rhodobacter capsulatus heat-resistant mutants; A52 and B41, with modified hydrogen production capacities, have been performed to identify mutations related to hydrogen production. In comparison with the reference strain DSM1710, the genomes of the mutants A52 and B41 contained 2137 and 2253 mutations, respectively. In the mutant B41 genome, mutations were characterized within the nifD, nifJ, glnD, nifB1, ccpA, hupD, dmsA, and cbbR1 genes. On the other hand; in the A52 mutant, mutations were characterized in the nifB2, rnfF, nifJ, cbbO, anfH, amt, moeA, and hupD genes. The effects of nitrogen metabolism and redox-related mutations were tested by quantitative reverse transcription PCR. The most promising mutation was found on the glnD gene of B41 strain with higher hydrogen production capacity.

AB - To overcome efficiency losses due to overheating in outdoor photobioreactors for microbial hydrogen production, heat-resistant microorganisms are desirable. In this study, the comparative whole genome sequencing analyses of two previously obtained Rhodobacter capsulatus heat-resistant mutants; A52 and B41, with modified hydrogen production capacities, have been performed to identify mutations related to hydrogen production. In comparison with the reference strain DSM1710, the genomes of the mutants A52 and B41 contained 2137 and 2253 mutations, respectively. In the mutant B41 genome, mutations were characterized within the nifD, nifJ, glnD, nifB1, ccpA, hupD, dmsA, and cbbR1 genes. On the other hand; in the A52 mutant, mutations were characterized in the nifB2, rnfF, nifJ, cbbO, anfH, amt, moeA, and hupD genes. The effects of nitrogen metabolism and redox-related mutations were tested by quantitative reverse transcription PCR. The most promising mutation was found on the glnD gene of B41 strain with higher hydrogen production capacity.

KW - Bacterial genomics

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