Chemically orthogonal three-patch microparticles

Compared to two-dimensional substrates, only a few methodologies exist for the spatially controlled decoration of three-dimensional objects, such as microparticles. Combining electrohydrodynamic co-jetting with synthetic polymer chemistry, we were able to create two- and three-patch microparticles displaying chemically orthogonal anchor groups on three distinct surface patches of the same particle. This approach takes advantage of a combination of novel chemically orthogonal polylactide-based polymers and their processing by electrohydrodynamic co-jetting to yield unprecedented multifunctional microparticles. Several micropatterned particles were fabricated displaying orthogonal click functionalities. Specifically, we demonstrate novel two- and three-patch particles. Multi-patch particles are highly sought after for their potential to present multiple distinct ligands in a directional manner. This work clearly establishes a viable route towards orthogonal reaction strategies on multivalent micropatterned particles. Patchwork family: Three-patch microparticles (see scheme) containing functionalized poly(lactide) derivatives in select compartments were fabricated through the electrohydrodynamic (EHD) co-jetting process. The microparticles were characterized by Raman confocal microspectroscopy and the bio-orthogonal surface modification of each patch was demonstrated.