Characterization of aquaporin Z-incorporated proteoliposomes with QCM-D

Reyhan Sengur-Tasdemir, Abdulhalim Kilic, Havva Esra Tutuncu, Tulay Ergon-Can, Nevin Gul-Karaguler, Esra Ates-Genceli, Fatma Nese Kok, Ismail Koyuncu*

*Bu çalışma için yazışmadan sorumlu yazar

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7 Atıf (Scopus)

Özet

Aquaporins have high water permeability and selectivity, which make them attractive to use in membrane applications for water purification and desalination. Due to their hydrophobic nature, they have to be embedded in cell membrane-like structures such as liposomes to form proteoliposomes. In this study, the effects of aquaporin Z (AqpZ) addition on liposome characteristics and behavior were investigated by evaluating the adsorption of proteoliposomes onto gold as reference substrate, polysulfone (PS) and polyacrylonitrile (PAN) spin-coated sensor surface as common nanofiltration membrane material by quartz crystal microbalance with dissipation. AqpZ incorporation into liposomes did not significantly change mean liposome diameter and surface charge, but the water permeability was increased up to almost sixfold. The results of viscoelastic modeling showed a stiffer membrane structure for the lipid bilayer of proteoliposomes compared to that of liposomes. Although liposomes and proteoliposomes were found to be adsorbed successfully on negatively charged PS and neutral PAN surfaces, the interaction strength and adsorption rate were found to be different for polymers with different surface properties, indicating the importance of the polymer for the stabilization of proteoliposomes. Findings of this study offer valuable information about the incorporation of proteoliposomes in membrane structures for the fabrication of nanofiltration membranes.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)133-142
Sayfa sayısı10
DergiSurface Innovations
Hacim7
Basın numarası2
DOI'lar
Yayın durumuYayınlandı - 15 Şub 2019

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Publisher Copyright:
© 2019 ICE Publishing: All rights reserved.

Finansman

The authors are grateful to the Scientific and Technological Research Council of Turkey for their financial support under a grant (Project Number 113Y359). The authors also thank Dr Melek Ozkan for her permission to use stopped-flow equipment.

FinansörlerFinansör numarası
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu113Y359

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