Breed effect on early cytokine mRNA expression in spleen and cecum of chickens with and without Salmonella enteritidis infection

Jennifer H. Cheeseman; Michael G. Kaiser; Ceren Ciraci; Pete Kaiser; Susan J. Lamont

doi:10.1016/j.dci.2006.04.001

Breed effect on early cytokine mRNA expression in spleen and cecum of chickens with and without Salmonella enteritidis infection

Jennifer H. Cheeseman
, Michael G. Kaiser
, Ceren Ciraci
, Pete Kaiser
, Susan J. Lamont^*

^*Bu çalışma için yazışmadan sorumlu yazar

Iowa State University
Pirbright Institute

Araştırma sonucu: Dergiye katkı › Makale › bilirkişi

91 Atıf (Scopus)

Özet

We examined mRNA expression of 11 genes: BAK, Bcl-x, Interferon [IFN]-γ, Interleukin [IL]-1β, IL-6, IL-10, IL-12α, IL-12β, IL-18, CXCLi2 [IL-8/CAF], and a MIP family chemokine, CCLi2, in the spleen and cecum of day-old chicks after oral inoculation with Salmonella enteritidis (SE) or medium. Three distinct chicken breeds (broiler, Fayoumi, and Leghorn) were evaluated for mRNA expression levels at 2 and 18 h post-inoculation using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). SE exposure significantly increased splenic IL-18 and IFN-γ expression. Breed effect was significant (P < 0.05) for CXCLi2, IL-10, IL-12α, and CCLi2 mRNA expression in the spleen, and for IL-12α, IL-12β, IL-18, and CCLi2 mRNA expression in the cecum. Generally, mRNA expression levels were higher in the spleen, and lower in the cecum, of Leghorns versus broilers. These results support a role for breed genetics influencing cytokine mRNA expression in young chickens and may potentially explain some generalized immune response differences between breeds.

Orijinal dil	İngilizce
Sayfa (başlangıç-bitiş)	52-60
Sayfa sayısı	9
Dergi	Developmental and Comparative Immunology
Hacim	31
Basın numarası	1
DOI'lar	https://doi.org/10.1016/j.dci.2006.04.001
Yayın durumu	Yayınlandı - 2007
Harici olarak yayınlandı	Evet

Finansman

The authors thank Jason Hasenstein and Huaijun Zhou for excellent technical assistance and sample preparation. This work was supported by National Research Initiative Grant no. 2004-35205-14234 from the USDA Cooperative State Research, Education and Extension Service; Research Grant IS-3021-98CR from BARD, the Binational Agriculture Research and Development Fund; and Animal Health, State of Iowa and the Iowa State University Center for Integrated Animal Genomics Funds.

Finansörler	Finansör numarası
Animal Health, State of Iowa
Binational Agriculture Research and Development Fund
Cooperative State Research, Education, and Extension Service	IS-3021-98CR
BARD

Belgeye Erişim

10.1016/j.dci.2006.04.001

Diğer Dosyalar ve Linkler

Link to publication in Scopus

Alıntı Yap

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title = "Breed effect on early cytokine mRNA expression in spleen and cecum of chickens with and without Salmonella enteritidis infection",

abstract = "We examined mRNA expression of 11 genes: BAK, Bcl-x, Interferon [IFN]-γ, Interleukin [IL]-1β, IL-6, IL-10, IL-12α, IL-12β, IL-18, CXCLi2 [IL-8/CAF], and a MIP family chemokine, CCLi2, in the spleen and cecum of day-old chicks after oral inoculation with Salmonella enteritidis (SE) or medium. Three distinct chicken breeds (broiler, Fayoumi, and Leghorn) were evaluated for mRNA expression levels at 2 and 18 h post-inoculation using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). SE exposure significantly increased splenic IL-18 and IFN-γ expression. Breed effect was significant (P < 0.05) for CXCLi2, IL-10, IL-12α, and CCLi2 mRNA expression in the spleen, and for IL-12α, IL-12β, IL-18, and CCLi2 mRNA expression in the cecum. Generally, mRNA expression levels were higher in the spleen, and lower in the cecum, of Leghorns versus broilers. These results support a role for breed genetics influencing cytokine mRNA expression in young chickens and may potentially explain some generalized immune response differences between breeds.",

keywords = "Cecum, Chicken, Cytokines, Genetics, Immunology, Salmonella enteritidis, Spleen",

author = "Cheeseman, \{Jennifer H.\} and Kaiser, \{Michael G.\} and Ceren Ciraci and Pete Kaiser and Lamont, \{Susan J.\}",

year = "2007",

doi = "10.1016/j.dci.2006.04.001",

language = "English",

volume = "31",

pages = "52--60",

journal = "Developmental and Comparative Immunology",

issn = "0145-305X",

publisher = "Elsevier Ltd",

number = "1",

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TY - JOUR

T1 - Breed effect on early cytokine mRNA expression in spleen and cecum of chickens with and without Salmonella enteritidis infection

AU - Cheeseman, Jennifer H.

AU - Kaiser, Michael G.

AU - Ciraci, Ceren

AU - Kaiser, Pete

AU - Lamont, Susan J.

PY - 2007

Y1 - 2007

N2 - We examined mRNA expression of 11 genes: BAK, Bcl-x, Interferon [IFN]-γ, Interleukin [IL]-1β, IL-6, IL-10, IL-12α, IL-12β, IL-18, CXCLi2 [IL-8/CAF], and a MIP family chemokine, CCLi2, in the spleen and cecum of day-old chicks after oral inoculation with Salmonella enteritidis (SE) or medium. Three distinct chicken breeds (broiler, Fayoumi, and Leghorn) were evaluated for mRNA expression levels at 2 and 18 h post-inoculation using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). SE exposure significantly increased splenic IL-18 and IFN-γ expression. Breed effect was significant (P < 0.05) for CXCLi2, IL-10, IL-12α, and CCLi2 mRNA expression in the spleen, and for IL-12α, IL-12β, IL-18, and CCLi2 mRNA expression in the cecum. Generally, mRNA expression levels were higher in the spleen, and lower in the cecum, of Leghorns versus broilers. These results support a role for breed genetics influencing cytokine mRNA expression in young chickens and may potentially explain some generalized immune response differences between breeds.

AB - We examined mRNA expression of 11 genes: BAK, Bcl-x, Interferon [IFN]-γ, Interleukin [IL]-1β, IL-6, IL-10, IL-12α, IL-12β, IL-18, CXCLi2 [IL-8/CAF], and a MIP family chemokine, CCLi2, in the spleen and cecum of day-old chicks after oral inoculation with Salmonella enteritidis (SE) or medium. Three distinct chicken breeds (broiler, Fayoumi, and Leghorn) were evaluated for mRNA expression levels at 2 and 18 h post-inoculation using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). SE exposure significantly increased splenic IL-18 and IFN-γ expression. Breed effect was significant (P < 0.05) for CXCLi2, IL-10, IL-12α, and CCLi2 mRNA expression in the spleen, and for IL-12α, IL-12β, IL-18, and CCLi2 mRNA expression in the cecum. Generally, mRNA expression levels were higher in the spleen, and lower in the cecum, of Leghorns versus broilers. These results support a role for breed genetics influencing cytokine mRNA expression in young chickens and may potentially explain some generalized immune response differences between breeds.

KW - Cecum

KW - Chicken

KW - Cytokines

KW - Genetics

KW - Immunology

KW - Salmonella enteritidis

KW - Spleen

UR - https://www.scopus.com/pages/publications/33748922946

U2 - 10.1016/j.dci.2006.04.001

DO - 10.1016/j.dci.2006.04.001

M3 - Article

C2 - 16762413

AN - SCOPUS:33748922946

SN - 0145-305X

VL - 31

SP - 52

EP - 60

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

IS - 1

ER -

Breed effect on early cytokine mRNA expression in spleen and cecum of chickens with and without Salmonella enteritidis infection

Özet

Finansman

Belgeye Erişim

Diğer Dosyalar ve Linkler

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