Antagonistic effect of chitinolytic Pseudomonas and Bacillus on growth of fungal hyphae and spores of aflatoxigenic Aspergillus flavus

Pervin Basaran Akocak*, John J. Churey, Randy W. Worobo

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: ???type-name???Makalebilirkişi

20 Atıf (Scopus)

Özet

Use of biological control methods has become a promising strategy to counteract deleterious fungal contaminations and substantial post harvest losses. One of the major biological control methods is found in the use of fungal cell wall-degrading enzymes such as chitinases, which hydrolyze the homopolymer of β-1,4-1inked N-acetylglucosamine residues exposed on the fungal cell surface. In this study, antagonistic effects of the chitinolytic enzyme producing isolates of Pseudomonas fluorescens PB27, Bacillus cereus B1 and Bacillus thuringiensis K1 against the aflatoxigenic fungus Aspergillus flavus were investigated. Germination of the spores of A. flavus was inhibited by up to nearly 20% after co-incubation with P. fluorescens PB27 for 72 h, whereas the fungal spores were less affected when incubated with either B. cereus B1 or B. thuringiensis K1. When P. fluorescens or its extracted extracellular enzyme preparation was sprayed on the surface of hazelnuts, the growth of A. flavus was reduced nearly 25%. Scanning electron microscopy observations of hyphae and spores revealed that these enzymes induce numerous ultrastructural morphological changes during spore germination and mycelium growth. Since chitinolytic enzymes have not been shown to be toxic to humans or the environment, based on the observations in this study, an understanding of the functional properties and antagonistic effect of this natural antifungal agent can be practical in the management of post-harvest losses caused by food pathogenic and aflatoxin producing fungi.

Orijinal dilİngilizce
Sayfa (başlangıç-bitiş)48-58
Sayfa sayısı11
DergiFood Bioscience
Hacim10
DOI'lar
Yayın durumuYayınlandı - 1 Haz 2015
Harici olarak yayınlandıEvet

Bibliyografik not

Publisher Copyright:
© 2015 Elsevier Ltd. All rights reserved.

Finansman

Pervin Basaran was supported by grants to work in Worobo Lab from the Turkish National Science Foundation (TUBITAK).2219

FinansörlerFinansör numarası
TUBITAK
Turkish National Science Foundation

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