An LED-based super resolution GPU implemented structured illumination microscope

Musa Aydin; Yiäit Uysalll; Ekin Özgönül; Berna Morova; Alper Kiraz

doi:10.1117/12.2544602

An LED-based super resolution GPU implemented structured illumination microscope

Musa Aydin
, Yiäit Uysalll
, Ekin Özgönül
, Berna Morova
, Alper Kiraz^*

^*Bu çalışma için yazışmadan sorumlu yazar

Fatih Sultan Mehmet Vakif Universitesi
Koc University

Araştırma çıktısı: Kitap/Rapor/Konferans Bildirisinde Bölüm › Konferans katkısı › Hakem

2 Atıf (Scopus)

Özet

Fluorescence imaging of sub-cellular structures with sizes below the diffraction limit is vital in understanding cel-lular processes. Relying on exciting the sample with different illumination patterns and image processing for the elimination of background fluorescence, Structured Illumination Microscopy (SIM) provides imaging capability beyond diffraction limit using relatively simple optical setups. Here, we present a laser-free, DLP projector-based, and GPU-implemented SIM super resolution microscope. Sub-diffractive biological structures were imaged with a lateral resolution of â1/4150 nm. The microscopy system is LED-based and entirely home-built which enables customizable operation at a low cost.

Orijinal dil	İngilizce
Ana bilgisayar yayını başlığı	Single Molecule Spectroscopy and Superresolution Imaging XIII
Editörler	Ingo Gregor, Felix Koberling, Rainer Erdmann
Yayınlayan	SPIE
ISBN (Elektronik)	9781510632554
DOI'lar	https://doi.org/10.1117/12.2544602
Yayın durumu	Yayınlandı - 2020
Harici olarak yayınlandı	Evet
Etkinlik	Single Molecule Spectroscopy and Superresolution Imaging XIII 2020 - San Francisco, United States Süre: 1 Şub 2020 → 2 Şub 2020

Yayın serisi

Adı	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Hacim	11246
ISSN (Basılı)	1605-7422

???event.eventtypes.event.conference???

???event.eventtypes.event.conference???	Single Molecule Spectroscopy and Superresolution Imaging XIII 2020
Ülke/Bölge	United States
Şehir	San Francisco
Periyot	1/02/20 → 2/02/20

Bibliyografik not

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

Belgeye Erişim

10.1117/12.2544602

Diğer Dosyalar ve Linkler

Link to publication in Scopus

Alıntı Yap

Aydin, M., Uysalll, Y., Özgönül, E., Morova, B., & Kiraz, A. (2020). An LED-based super resolution GPU implemented structured illumination microscope. In I. Gregor, F. Koberling, & R. Erdmann (Eds.), Single Molecule Spectroscopy and Superresolution Imaging XIII Makale 1124610 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Hac. 11246). SPIE. https://doi.org/10.1117/12.2544602

@inproceedings{a800c1a7f6d74423a1d123871d65df03,

title = "An LED-based super resolution GPU implemented structured illumination microscope",

abstract = "Fluorescence imaging of sub-cellular structures with sizes below the diffraction limit is vital in understanding cel-lular processes. Relying on exciting the sample with different illumination patterns and image processing for the elimination of background fluorescence, Structured Illumination Microscopy (SIM) provides imaging capability beyond diffraction limit using relatively simple optical setups. Here, we present a laser-free, DLP projector-based, and GPU-implemented SIM super resolution microscope. Sub-diffractive biological structures were imaged with a lateral resolution of {\^a}1/4150 nm. The microscopy system is LED-based and entirely home-built which enables customizable operation at a low cost.",

keywords = "DMD, GPU, Structured Illumination Microscopy (SIM), Super Resolution Microscopy",

author = "Musa Aydin and Yi{\"a}it Uysalll and Ekin {\"O}zg{\"o}n{\"u}l and Berna Morova and Alper Kiraz",

note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Single Molecule Spectroscopy and Superresolution Imaging XIII 2020 ; Conference date: 01-02-2020 Through 02-02-2020",

year = "2020",

doi = "10.1117/12.2544602",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Ingo Gregor and Felix Koberling and Rainer Erdmann",

booktitle = "Single Molecule Spectroscopy and Superresolution Imaging XIII",

address = "United States",

}

Aydin, M, Uysalll, Y, Özgönül, E, Morova, B & Kiraz, A 2020, An LED-based super resolution GPU implemented structured illumination microscope. in I Gregor, F Koberling & R Erdmann (eds), Single Molecule Spectroscopy and Superresolution Imaging XIII., 1124610, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, hac. 11246, SPIE, Single Molecule Spectroscopy and Superresolution Imaging XIII 2020, San Francisco, United States, 1/02/20. https://doi.org/10.1117/12.2544602

An LED-based super resolution GPU implemented structured illumination microscope. / Aydin, Musa; Uysalll, Yiäit; Özgönül, Ekin et al.
Single Molecule Spectroscopy and Superresolution Imaging XIII. ed. / Ingo Gregor; Felix Koberling; Rainer Erdmann. SPIE, 2020. 1124610 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Hac. 11246).

Araştırma çıktısı: Kitap/Rapor/Konferans Bildirisinde Bölüm › Konferans katkısı › Hakem

TY - GEN

T1 - An LED-based super resolution GPU implemented structured illumination microscope

AU - Aydin, Musa

AU - Uysalll, Yiäit

AU - Özgönül, Ekin

AU - Morova, Berna

AU - Kiraz, Alper

PY - 2020

Y1 - 2020

N2 - Fluorescence imaging of sub-cellular structures with sizes below the diffraction limit is vital in understanding cel-lular processes. Relying on exciting the sample with different illumination patterns and image processing for the elimination of background fluorescence, Structured Illumination Microscopy (SIM) provides imaging capability beyond diffraction limit using relatively simple optical setups. Here, we present a laser-free, DLP projector-based, and GPU-implemented SIM super resolution microscope. Sub-diffractive biological structures were imaged with a lateral resolution of â1/4150 nm. The microscopy system is LED-based and entirely home-built which enables customizable operation at a low cost.

AB - Fluorescence imaging of sub-cellular structures with sizes below the diffraction limit is vital in understanding cel-lular processes. Relying on exciting the sample with different illumination patterns and image processing for the elimination of background fluorescence, Structured Illumination Microscopy (SIM) provides imaging capability beyond diffraction limit using relatively simple optical setups. Here, we present a laser-free, DLP projector-based, and GPU-implemented SIM super resolution microscope. Sub-diffractive biological structures were imaged with a lateral resolution of â1/4150 nm. The microscopy system is LED-based and entirely home-built which enables customizable operation at a low cost.

KW - DMD

KW - GPU

KW - Structured Illumination Microscopy (SIM)

KW - Super Resolution Microscopy

UR - https://www.scopus.com/pages/publications/85082165676

U2 - 10.1117/12.2544602

DO - 10.1117/12.2544602

M3 - Conference contribution

AN - SCOPUS:85082165676

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Single Molecule Spectroscopy and Superresolution Imaging XIII

A2 - Gregor, Ingo

A2 - Koberling, Felix

A2 - Erdmann, Rainer

PB - SPIE

T2 - Single Molecule Spectroscopy and Superresolution Imaging XIII 2020

Y2 - 1 February 2020 through 2 February 2020

ER -

An LED-based super resolution GPU implemented structured illumination microscope

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