Adsorption and desorption studies of lysozyme by Fe₃O₄-polymer nanocomposite via fluorescence spectroscopy

The work have been undertaken in this study is to synthesis and characterize Fe₃O₄-polymer nanocomposites which are having different morphological properties. Also, investigation of the adsorption and desorption behaviour of lysozyme onto Fe₃O₄-polymer nanocomposites have been studied. Fe₃O₄ nanoparticles, synthesized by in situ in polyacrylamide hydrogels, show super-paramagnetic behaviour and saturation magnetization of composite material have been tuned by changing the hydrogel conformation. Adsorption and desorption studies of lysozyme were followed by using pure water at room temperature via fluorescence measurements. Fluorescence measurements showed that, the composite materials adsorbed lysozyme molecules less than 20 s and higher monomer concentration of composite materials cause faster adsorption. Besides, structure of lysozyme molecules were not changed during the adsorption and desorption. As a result Fe₃O₄-polymer nanocomposites could be used for drug delivery, protein separation and PAAm gels could be used for synthesis of magnetic composites with varying magnetic properties.