A new highly Selective, sensitive and NIR spectrophotometric probe based on A₂B₂-Type of unsymmetrical phthalocyanine for hazardous Be²⁺ recognition

The aim of this work is to construct a new A₂B₂-type of unsymmetrical and ratiometric phthalocyanine (Pc) based-probe O-A₂B₂ZnPc to provide an effective solution to critical inadequacy to be experienced for the detection of hazardous Be²⁺. O-A₂B₂ZnPc enabling strong absorption and emission in Near-Infrared region (λ_abs-λ_em wavelengths of 694–712 nm) showed excellent selectivity and sensitivity toward Be²⁺ among competitive metal ions by both spectrophotometric and fluorometric methods. The probe with oligomeric Pc form in THF was degraded with the addition of aqueous Be²⁺ and arranged to J-aggregation form, resulting in a remarkably diminishing in Q-band at 694 nm as well as a new band formation at 746 nm, and a considerably decreasing in fluorescence emission. The probe has superior features for the determination of Be²⁺ such as high quantum efficiency and photochemical stability, rapid response (1 s), high selectivity and very low Limit of Detection (0.26 ppb and 1.5 ppb) for UV–Vis and fluorescence, respectively which are quite good values according to the permissible amount of Be²⁺ (4 ppb) in water as specified by World Health Organization. O-A₂B₂ZnPc can be shown among the best performing probes with its unique properties according to previous studies in the literature. In addition, the geometrical and spectral features of the O-A₂B₂ZnPc were analyzed in detail by DFT calculations.