A microfluidic 3D cell culture system for drug discovery studies

Emre Altinagac; Volkan Yalman; Murat Demirbilek; Huseyin Kizil

doi:10.11159/nddte19.117

A microfluidic 3D cell culture system for drug discovery studies

Emre Altinagac
, Volkan Yalman
, Murat Demirbilek
, Huseyin Kizil

Metalurji ve Malzeme Müh.Böl.

Istanbul Technical University
Yildiz Technical University
Hacettepe University

Araştırma çıktısı: Dergi yayını › Konferans makalesi › Hakem

Özet

In this paper, a microfluidic platform with bottom and upper microchannels separated by a polycarbonate membrane is studied for three-dimensional cell culture applications. Microfluidic chips were designed and fabricated using standard soft lithography and micromolding methods. The effect of surface modification by NaOH of polycarbonate membranes on the cell viability was investigated. Mouse fibroblast cells were cultured on modified and non-modified membrane integrated microfluidic chips. On 2nd, 3rd and 4th day of cultivation, cell concentrations were recorded and it was found that the cell concentration on modified membranes was always higher at all time intervals, and after four days, the cell concentration on the modified membrane was about 55 % higher than that of unmodified case.

Orijinal dil	İngilizce
Makale numarası	117
Dergi	World Congress on Recent Advances in Nanotechnology
DOI'lar	https://doi.org/10.11159/nddte19.117
Yayın durumu	Yayınlandı - 2019
Etkinlik	4th World Congress on Recent Advances in Nanotechnology, RAN 2019 - Süre: 14 Nis 2019 → 16 Nis 2019

Bibliyografik not

Publisher Copyright:
© 2019, Avestia Publishing.

Finansman

The authors would like to acknowledge the financial support provided by the Scientific and Technological Research Council of Turkey (TUBITAK) under Grant No.117M296.

Finansörler	Finansör numarası
TUBITAK	117M296
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu

Belgeye Erişim

10.11159/nddte19.117

Diğer Dosyalar ve Linkler

Link to publication in Scopus

Alıntı Yap

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title = "A microfluidic 3D cell culture system for drug discovery studies",

abstract = "In this paper, a microfluidic platform with bottom and upper microchannels separated by a polycarbonate membrane is studied for three-dimensional cell culture applications. Microfluidic chips were designed and fabricated using standard soft lithography and micromolding methods. The effect of surface modification by NaOH of polycarbonate membranes on the cell viability was investigated. Mouse fibroblast cells were cultured on modified and non-modified membrane integrated microfluidic chips. On 2nd, 3rd and 4th day of cultivation, cell concentrations were recorded and it was found that the cell concentration on modified membranes was always higher at all time intervals, and after four days, the cell concentration on the modified membrane was about 55 \% higher than that of unmodified case.",

keywords = "3D cell culture, Microfluidics, PC membrane, PDMS microchannel, Surface modification",

author = "Emre Altinagac and Volkan Yalman and Murat Demirbilek and Huseyin Kizil",

note = "Publisher Copyright: {\textcopyright} 2019, Avestia Publishing.; 4th World Congress on Recent Advances in Nanotechnology, RAN 2019 ; Conference date: 14-04-2019 Through 16-04-2019",

year = "2019",

doi = "10.11159/nddte19.117",

language = "English",

journal = "World Congress on Recent Advances in Nanotechnology",

issn = "2371-5308",

publisher = "Avestia Publishing",

}

TY - JOUR

T1 - A microfluidic 3D cell culture system for drug discovery studies

AU - Altinagac, Emre

AU - Yalman, Volkan

AU - Demirbilek, Murat

AU - Kizil, Huseyin

PY - 2019

Y1 - 2019

N2 - In this paper, a microfluidic platform with bottom and upper microchannels separated by a polycarbonate membrane is studied for three-dimensional cell culture applications. Microfluidic chips were designed and fabricated using standard soft lithography and micromolding methods. The effect of surface modification by NaOH of polycarbonate membranes on the cell viability was investigated. Mouse fibroblast cells were cultured on modified and non-modified membrane integrated microfluidic chips. On 2nd, 3rd and 4th day of cultivation, cell concentrations were recorded and it was found that the cell concentration on modified membranes was always higher at all time intervals, and after four days, the cell concentration on the modified membrane was about 55 % higher than that of unmodified case.

AB - In this paper, a microfluidic platform with bottom and upper microchannels separated by a polycarbonate membrane is studied for three-dimensional cell culture applications. Microfluidic chips were designed and fabricated using standard soft lithography and micromolding methods. The effect of surface modification by NaOH of polycarbonate membranes on the cell viability was investigated. Mouse fibroblast cells were cultured on modified and non-modified membrane integrated microfluidic chips. On 2nd, 3rd and 4th day of cultivation, cell concentrations were recorded and it was found that the cell concentration on modified membranes was always higher at all time intervals, and after four days, the cell concentration on the modified membrane was about 55 % higher than that of unmodified case.

KW - 3D cell culture

KW - Microfluidics

KW - PC membrane

KW - PDMS microchannel

KW - Surface modification

UR - https://www.scopus.com/pages/publications/85086673406

U2 - 10.11159/nddte19.117

DO - 10.11159/nddte19.117

M3 - Conference article

AN - SCOPUS:85086673406

SN - 2371-5308

JO - World Congress on Recent Advances in Nanotechnology

JF - World Congress on Recent Advances in Nanotechnology

M1 - 117

T2 - 4th World Congress on Recent Advances in Nanotechnology, RAN 2019

Y2 - 14 April 2019 through 16 April 2019

ER -

A microfluidic 3D cell culture system for drug discovery studies

Özet

Bibliyografik not

Finansman

Belgeye Erişim

Diğer Dosyalar ve Linkler

Parmak izi

Alıntı Yap