A leucine aminopeptidase activatable photosensitizer for cancer cell selective photodynamic therapy action

Busra Arslan, Kubra Bilici*, Gozde Demirci, Toghrul Almammadov, Minahil Khan, Alphan Sennaroglu, Havva Yagci Acar, Safacan Kolemen*

*Bu çalışma için yazışmadan sorumlu yazar

Araştırma sonucu: Dergiye katkıMakalebilirkişi

27 Atıf (Scopus)

Özet

Activity based photosensitizers (PS) continue to attract great attention as they enable selective photodynamic therapy action on cancer cells while sparing normal cells even under light irradiation. Sensitivity to specific enzymes that are differentially overexpressed in cancer cells is crucial in the design of activatable PSs. In this direction, we report here, for the first time, a leucine aminopeptidase (LAP) activatable PDT agent (HCL), which is a red-shifted, water soluble and photostable brominated hemicyanine derivative. HCL was activated by endogenous LAP enzyme selectively in A549 (lung) and HCT116 (colon) cancer cells containing high LAP levels and induced effective photocytotoxicity with negligible dark toxicity. Furthermore, the fluorescence of the parent bromo-hemicyanine core was restored upon LAP-based activation in cancer cells. On the other side, no remarkable phototoxicity or fluorescence turn-on was detected in healthy L929 cells. Thus, HCL serves as an effective and tumour associated LAP-sensitive phototheranostic agent. We believe different cancer-associated analytes can be utilized in combination with near-IR absorbing scaffolds in the scope of activatable PDT designs to enrich the tumour-selective PS arsenal.

Orijinal dilİngilizce
Makale numarası109735
DergiDyes and Pigments
Hacim195
DOI'lar
Yayın durumuYayınlandı - Kas 2021
Harici olarak yayınlandıEvet

Bibliyografik not

Publisher Copyright:
© 2021 Elsevier Ltd

Finansman

This work was partially supported by the BAGEP Award of the Science Academy and Koç University Seed Funding.

FinansörlerFinansör numarası
Bilim Akademisi

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