Abstract
The suitability of the vacancy affinity capillary electrophoretic method (VACE) for study of displacement of a target drag from a protein by simultaneously administered drags was investigated. As test system, the displacement of warfarin from bovine serum albumin (BSA) by furosemide and phenylbutazone was selected. It appears that the displacement can be observed well from the shift of the actual mobility of warfarin when a displacer drug is added. Also, the competitive action of the displacer drugs (affinity for BSA) is clearly visible. The VACE method seems to be attractive for rapid assesssment of information about the competitive properties of coadministered compounds.
| Original language | English |
|---|---|
| Pages (from-to) | 205-210 |
| Number of pages | 6 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 710 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 12 Jun 1998 |
| Externally published | Yes |
Keywords
- Protein-drug binding
- Vacancy affinity capillary electrophoresis