Use of Echium oil fatty acids and tricaprylin as substrates of enzymatic interesterification for the production of structured lipids

A. Yüksel; N. Şahin-Yeşilçubuk

doi:10.3989/gya.0996171

Use of Echium oil fatty acids and tricaprylin as substrates of enzymatic interesterification for the production of structured lipids

A. Yüksel, N. Şahin-Yeşilçubuk^*

^*Corresponding author for this work

Department of Food Engineering

Istanbul Technical University

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Structured lipids (SLs) were produced from the free fatty acids of Echium oil and tricaprylin by enzymatic acidolysis reactions. Lipozyme^® RM IM, immobilized sn-1,3 specific lipase was used in the enzymatic reactions. In order to optimize the incorporation of stearidonic acid (SDA), three factors were chosen [Reaction temperature (50-60 ºC), reaction time (6-12 hour) and substrate molar ratio (3-6 mol/mol (total free fatty acids/ tricaprylin)] for the application of response surface methodology (RSM) using a central composite circumscribed design (CCC) with five levels. The optimum temperature, time and substrate molar ratio obtained from the models were 60 ºC; 6 h, 6 mol/mol, respectively. Furthermore, SLs with 6.2% SDA content at sn-2 position were produced by scaling up the process. SL was obtained with nearly 78-79% of long-chain fatty acids at the sn-2 position. According to the melting profile analysis, the melting peaks of tricaprylin and Echium oil were sharper and narrower while the SL had more broadened peaks.

Original language	English
Article number	e236
Journal	Grasas y Aceites
Volume	69
Issue number	1
DOIs	https://doi.org/10.3989/gya.0996171
Publication status	Published - 1 Jan 2018

Bibliographical note

Publisher Copyright:
© 2018 CSIC.

Funding

grasos libres de aceite de Echium y tricaprilina mediante reacciones de acidolisis enzimática. Se usó Lipozyme® RM IM, lipasa inmovilizada específica de sn-1,3 en las reacciones enzimáticas. Para optimizar la incorporación de ácido estearidónico (SDA), se eligieron tres factores [Temperatura de reacción (50-60 ºC), tiempo de reacción (6-12 horas) y proporción molar del sustrato (3-6 mol/mol (total de ácidos grasos libres) / tricaprylin)] para la aplicación de la metodología de superficie de respuesta (RSM) mediante el uso de un diseño compuesto central circunscrito (CCC) con cinco niveles. La relación óptima de temperatura, tiempo y sustrato obtenida de los modelos fue 60 ºC; 6 h, 6 mol/mol. Además, se produjeron SL con un contenido de SDA del 6,2% en la posición sn-2 mediante el proceso de escalamiento, SL se obtuvo con casi 78-79% de ácidos grasos de cadena larga en la posición sn-2. Según el análisis del perfil de fusión, los picos de fusión de la tricaprilina y el aceite de Echium fueron más agudos y estrechos, mientras que los SL tuvieron picos más amplios.

Funders	Funder number
Total
Childhood Cancer Canada

Keywords

Echium oil
Enzymatic acidolysis
Lipozyme® RM IM
Response surface methodology
Stearidonic acid
Tricaprylin

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10.3989/gya.0996171

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title = "Use of Echium oil fatty acids and tricaprylin as substrates of enzymatic interesterification for the production of structured lipids",

abstract = "Structured lipids (SLs) were produced from the free fatty acids of Echium oil and tricaprylin by enzymatic acidolysis reactions. Lipozyme{\textregistered} RM IM, immobilized sn-1,3 specific lipase was used in the enzymatic reactions. In order to optimize the incorporation of stearidonic acid (SDA), three factors were chosen [Reaction temperature (50-60 ºC), reaction time (6-12 hour) and substrate molar ratio (3-6 mol/mol (total free fatty acids/ tricaprylin)] for the application of response surface methodology (RSM) using a central composite circumscribed design (CCC) with five levels. The optimum temperature, time and substrate molar ratio obtained from the models were 60 ºC; 6 h, 6 mol/mol, respectively. Furthermore, SLs with 6.2% SDA content at sn-2 position were produced by scaling up the process. SL was obtained with nearly 78-79% of long-chain fatty acids at the sn-2 position. According to the melting profile analysis, the melting peaks of tricaprylin and Echium oil were sharper and narrower while the SL had more broadened peaks.",

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N2 - Structured lipids (SLs) were produced from the free fatty acids of Echium oil and tricaprylin by enzymatic acidolysis reactions. Lipozyme® RM IM, immobilized sn-1,3 specific lipase was used in the enzymatic reactions. In order to optimize the incorporation of stearidonic acid (SDA), three factors were chosen [Reaction temperature (50-60 ºC), reaction time (6-12 hour) and substrate molar ratio (3-6 mol/mol (total free fatty acids/ tricaprylin)] for the application of response surface methodology (RSM) using a central composite circumscribed design (CCC) with five levels. The optimum temperature, time and substrate molar ratio obtained from the models were 60 ºC; 6 h, 6 mol/mol, respectively. Furthermore, SLs with 6.2% SDA content at sn-2 position were produced by scaling up the process. SL was obtained with nearly 78-79% of long-chain fatty acids at the sn-2 position. According to the melting profile analysis, the melting peaks of tricaprylin and Echium oil were sharper and narrower while the SL had more broadened peaks.

AB - Structured lipids (SLs) were produced from the free fatty acids of Echium oil and tricaprylin by enzymatic acidolysis reactions. Lipozyme® RM IM, immobilized sn-1,3 specific lipase was used in the enzymatic reactions. In order to optimize the incorporation of stearidonic acid (SDA), three factors were chosen [Reaction temperature (50-60 ºC), reaction time (6-12 hour) and substrate molar ratio (3-6 mol/mol (total free fatty acids/ tricaprylin)] for the application of response surface methodology (RSM) using a central composite circumscribed design (CCC) with five levels. The optimum temperature, time and substrate molar ratio obtained from the models were 60 ºC; 6 h, 6 mol/mol, respectively. Furthermore, SLs with 6.2% SDA content at sn-2 position were produced by scaling up the process. SL was obtained with nearly 78-79% of long-chain fatty acids at the sn-2 position. According to the melting profile analysis, the melting peaks of tricaprylin and Echium oil were sharper and narrower while the SL had more broadened peaks.

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KW - Lipozyme® RM IM

KW - Response surface methodology

KW - Stearidonic acid

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Use of Echium oil fatty acids and tricaprylin as substrates of enzymatic interesterification for the production of structured lipids

Abstract

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Keywords

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