TY - JOUR
T1 - Toluene inhibition on an anaerobic reactor sludge in terms of potential activity and composition of acetoclastic methanogens
AU - Ince, Orhan
AU - Kolukirik, Mustafa
AU - Cetecioglu, Zeynep
AU - Eyice, Ozge
AU - Inceoglu, Ozgul
AU - Ince, Bahar
PY - 2009
Y1 - 2009
N2 - The aim of this study was to determine the effect of toluene on an anaerobic sludge taken from a full-scale upflow anaerobic sludge blanket (UASB) reactor in terms of potential activity and composition of acetoclastic methanogens. Specific methanogenic activity (SMA) test results showed that 5%, 9.5%, 14%, 24%, 29%, 38% and 62% inhibition occurred in the potential methane production (PMP) rate of the sludge at toluene concentrations of 0.1 mM, 0.2 mM, 0.3 mM, 0.4 mM, 0.5 mM, 0.6 mM and 1 mM, respectively. Fluorescence in situ hybridization (FISH) results showed that relative abundance of archaeal cells was approx. 19% throughout the SMA tests. The anaerobic sludge was dominated by acetoclastic genus Methanosaeta which were slightly affected by increasing toluene concentrations do not have any effect on relative abundance of Methanosaeta spp., which was between 73% ± 1.6 and 68% ± 2.1 of the archaeal population.
AB - The aim of this study was to determine the effect of toluene on an anaerobic sludge taken from a full-scale upflow anaerobic sludge blanket (UASB) reactor in terms of potential activity and composition of acetoclastic methanogens. Specific methanogenic activity (SMA) test results showed that 5%, 9.5%, 14%, 24%, 29%, 38% and 62% inhibition occurred in the potential methane production (PMP) rate of the sludge at toluene concentrations of 0.1 mM, 0.2 mM, 0.3 mM, 0.4 mM, 0.5 mM, 0.6 mM and 1 mM, respectively. Fluorescence in situ hybridization (FISH) results showed that relative abundance of archaeal cells was approx. 19% throughout the SMA tests. The anaerobic sludge was dominated by acetoclastic genus Methanosaeta which were slightly affected by increasing toluene concentrations do not have any effect on relative abundance of Methanosaeta spp., which was between 73% ± 1.6 and 68% ± 2.1 of the archaeal population.
KW - Acetoclastic methanogenic activity
KW - FISH
KW - Inhibition
KW - Methanosaeta spp.
KW - Toluene
UR - http://www.scopus.com/inward/record.url?scp=77952560601&partnerID=8YFLogxK
U2 - 10.1080/10934520903263470
DO - 10.1080/10934520903263470
M3 - Article
C2 - 20183513
AN - SCOPUS:77952560601
SN - 1093-4529
VL - 44
SP - 1551
EP - 1556
JO - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
JF - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
IS - 14
ER -