Time-dependent leaf proteome alterations of Brachypodium distachyon in response to drought stress

Ozge Tatli, Bahar Sogutmaz Ozdemir, Gizem Dinler Doganay*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Key message: For the first time, a comprehensive proteome analysis was conducted on Brachypodium leaves under drought stress. Gradual changes in response to drought stress were monitored. Abstract: Drought is one of the major stress factors that dramatically affect the agricultural productivity worldwide. Improving the yield under drought is an urgent challenge in agriculture. Brachypodium distachyon is a model species for monocot plants such as wheat, barley and several potential biofuel grasses. In the current study, a comprehensive proteome analysis was conducted on Brachypodium leaves under different levels of drought application. To screen gradual changes upon drought, Brachypodium leaves subjected to drought for 4, 8 and 12 days were collected for each treatment day and relative water content of the leaves was measured for each time point. Cellular responses of Brachypodium were investigated through a proteomic approach involving two dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry (MS). Among 497 distinct spots in Brachypodium protein repertoire, a total of 13 differentially expressed proteins (DEPs) were identified as responsive to drought by mass spectrometry and classified according to their functions using bioinformatics tools. The biological functions of DEPs included roles in photosynthesis, protein folding, antioxidant mechanism and metabolic processes, which responded differentially at each time point of drought treatment. To examine further transcriptional expression of the genes that code identified protein, quantitative real time PCR (qRT-PCR) was performed. Identified proteins will contribute to the studies involving development of drought-resistant crop species and lead to the delineation of molecular mechanisms in drought response.

Original languageEnglish
Pages (from-to)609-623
Number of pages15
JournalPlant Molecular Biology
Volume94
Issue number6
DOIs
Publication statusPublished - 1 Aug 2017

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media B.V.

Funding

We would like to thank Kocaeli University, Faculty of Medicine, Department of Medical Biology for technical support and MALDI-TOF/TOF analysis. This work was supported by TUBITAK Research Funds (Project No: 115Z087). Brachypodium seeds used in this study were kindly provided by Prof. Dr. Metin Tuna, Namık Kemal University, Department of Field Crops, Tekirdağ, Turkey.

FundersFunder number
TUBITAK Research Funds115Z087
Kocaeli Üniversitesi
Tekirdağ Namık Kemal Üniversitesi

    Keywords

    • 2D-DIGE
    • Brachypodium distachyon
    • Drought stress
    • MALDI-TOF
    • Proteome analysis
    • Two-dimensional gel electrophoresis

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