Abstract
Key message: For the first time, a comprehensive proteome analysis was conducted on Brachypodium leaves under drought stress. Gradual changes in response to drought stress were monitored. Abstract: Drought is one of the major stress factors that dramatically affect the agricultural productivity worldwide. Improving the yield under drought is an urgent challenge in agriculture. Brachypodium distachyon is a model species for monocot plants such as wheat, barley and several potential biofuel grasses. In the current study, a comprehensive proteome analysis was conducted on Brachypodium leaves under different levels of drought application. To screen gradual changes upon drought, Brachypodium leaves subjected to drought for 4, 8 and 12 days were collected for each treatment day and relative water content of the leaves was measured for each time point. Cellular responses of Brachypodium were investigated through a proteomic approach involving two dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry (MS). Among 497 distinct spots in Brachypodium protein repertoire, a total of 13 differentially expressed proteins (DEPs) were identified as responsive to drought by mass spectrometry and classified according to their functions using bioinformatics tools. The biological functions of DEPs included roles in photosynthesis, protein folding, antioxidant mechanism and metabolic processes, which responded differentially at each time point of drought treatment. To examine further transcriptional expression of the genes that code identified protein, quantitative real time PCR (qRT-PCR) was performed. Identified proteins will contribute to the studies involving development of drought-resistant crop species and lead to the delineation of molecular mechanisms in drought response.
Original language | English |
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Pages (from-to) | 609-623 |
Number of pages | 15 |
Journal | Plant Molecular Biology |
Volume | 94 |
Issue number | 6 |
DOIs | |
Publication status | Published - 1 Aug 2017 |
Bibliographical note
Publisher Copyright:© 2017, Springer Science+Business Media B.V.
Funding
We would like to thank Kocaeli University, Faculty of Medicine, Department of Medical Biology for technical support and MALDI-TOF/TOF analysis. This work was supported by TUBITAK Research Funds (Project No: 115Z087). Brachypodium seeds used in this study were kindly provided by Prof. Dr. Metin Tuna, Namık Kemal University, Department of Field Crops, Tekirdağ, Turkey.
Funders | Funder number |
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TUBITAK Research Funds | 115Z087 |
Kocaeli Üniversitesi | |
Tekirdağ Namık Kemal Üniversitesi |
Keywords
- 2D-DIGE
- Brachypodium distachyon
- Drought stress
- MALDI-TOF
- Proteome analysis
- Two-dimensional gel electrophoresis