The association of variations in TLR genes and spontaneous immune control of hepatitis B virus

Seyma Katrinli; Adil Nigdelioglu; Kamil Ozdil; Gizem Dinler-Doganay; Levent Doganay

doi:10.1016/j.clinre.2017.10.002

The association of variations in TLR genes and spontaneous immune control of hepatitis B virus

Seyma Katrinli, Adil Nigdelioglu, Kamil Ozdil, Gizem Dinler-Doganay, Levent Doganay^*

^*Corresponding author for this work

Department of Molecular Biology and Genetics

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background: Toll-like receptors (TLRs) are suspected to play a critical role in liver diseases and the progression of chronic hepatitis B (CHB) infection. In this study, we investigated the possible association between TLRs and hepatitis B virus (HBV) infection chronicity in Turkish population. Methods: TLR4 (+896 A→G) (rs4986790), TLR5 (+1846 T→C) (rs5744174) and TLR9 (−1237T→C) (rs5743836) polymorphisms were screened in 131 CHB patient and 168 individuals by polymerase chain reaction (PCR) – restriction fragment length polymorphism (RFLP) technique. Results: Of the screened polymorphisms, TT genotype of the missense variant TLR5 (rs5744174) (NM_003268.5:c.1846T > C (p.Phe616Leu) is significantly more frequent in the control group than CHB patients (P < 0.001), presence of TT genotype of the upstream variant TLR9 (rs5743836) (NM_017442.3:c.−1237T > C) is more frequent in CHB group (P = 0.043). However, no significant association was found for the missense variant TLR4 (rs4986790) NM_138554.4:c.896A > G (p.Asp299Gly) polymorphism and CHB in Turkish population. Conclusions: From all three analyzed SNPs association of TLR5 (rs5744174) with CHB is the most significant. Since TLR5 is associated with interferon-γ production, a high frequency of TT at rs5744174 in controls subjects suggests that it represents a protective genotype against CHB plausibly associated with an increased interferon-γ production.

Original language	English
Pages (from-to)	139-144
Number of pages	6
Journal	Clinics and Research in Hepatology and Gastroenterology
Volume	42
Issue number	2
DOIs	https://doi.org/10.1016/j.clinre.2017.10.002
Publication status	Published - Apr 2018

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Masson SAS

Keywords

Chronic hepatitis B
PCR-RFLP
Polymorphism
Seroconversion
Toll-like receptors

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.clinre.2017.10.002

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title = "The association of variations in TLR genes and spontaneous immune control of hepatitis B virus",

abstract = "Background: Toll-like receptors (TLRs) are suspected to play a critical role in liver diseases and the progression of chronic hepatitis B (CHB) infection. In this study, we investigated the possible association between TLRs and hepatitis B virus (HBV) infection chronicity in Turkish population. Methods: TLR4 (+896 A→G) (rs4986790), TLR5 (+1846 T→C) (rs5744174) and TLR9 (−1237T→C) (rs5743836) polymorphisms were screened in 131 CHB patient and 168 individuals by polymerase chain reaction (PCR) – restriction fragment length polymorphism (RFLP) technique. Results: Of the screened polymorphisms, TT genotype of the missense variant TLR5 (rs5744174) (NM_003268.5:c.1846T > C (p.Phe616Leu) is significantly more frequent in the control group than CHB patients (P < 0.001), presence of TT genotype of the upstream variant TLR9 (rs5743836) (NM_017442.3:c.−1237T > C) is more frequent in CHB group (P = 0.043). However, no significant association was found for the missense variant TLR4 (rs4986790) NM_138554.4:c.896A > G (p.Asp299Gly) polymorphism and CHB in Turkish population. Conclusions: From all three analyzed SNPs association of TLR5 (rs5744174) with CHB is the most significant. Since TLR5 is associated with interferon-γ production, a high frequency of TT at rs5744174 in controls subjects suggests that it represents a protective genotype against CHB plausibly associated with an increased interferon-γ production.",

keywords = "Chronic hepatitis B, PCR-RFLP, Polymorphism, Seroconversion, Toll-like receptors",

author = "Seyma Katrinli and Adil Nigdelioglu and Kamil Ozdil and Gizem Dinler-Doganay and Levent Doganay",

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doi = "10.1016/j.clinre.2017.10.002",

language = "English",

volume = "42",

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T1 - The association of variations in TLR genes and spontaneous immune control of hepatitis B virus

AU - Katrinli, Seyma

AU - Nigdelioglu, Adil

AU - Ozdil, Kamil

AU - Dinler-Doganay, Gizem

AU - Doganay, Levent

PY - 2018/4

Y1 - 2018/4

N2 - Background: Toll-like receptors (TLRs) are suspected to play a critical role in liver diseases and the progression of chronic hepatitis B (CHB) infection. In this study, we investigated the possible association between TLRs and hepatitis B virus (HBV) infection chronicity in Turkish population. Methods: TLR4 (+896 A→G) (rs4986790), TLR5 (+1846 T→C) (rs5744174) and TLR9 (−1237T→C) (rs5743836) polymorphisms were screened in 131 CHB patient and 168 individuals by polymerase chain reaction (PCR) – restriction fragment length polymorphism (RFLP) technique. Results: Of the screened polymorphisms, TT genotype of the missense variant TLR5 (rs5744174) (NM_003268.5:c.1846T > C (p.Phe616Leu) is significantly more frequent in the control group than CHB patients (P < 0.001), presence of TT genotype of the upstream variant TLR9 (rs5743836) (NM_017442.3:c.−1237T > C) is more frequent in CHB group (P = 0.043). However, no significant association was found for the missense variant TLR4 (rs4986790) NM_138554.4:c.896A > G (p.Asp299Gly) polymorphism and CHB in Turkish population. Conclusions: From all three analyzed SNPs association of TLR5 (rs5744174) with CHB is the most significant. Since TLR5 is associated with interferon-γ production, a high frequency of TT at rs5744174 in controls subjects suggests that it represents a protective genotype against CHB plausibly associated with an increased interferon-γ production.

AB - Background: Toll-like receptors (TLRs) are suspected to play a critical role in liver diseases and the progression of chronic hepatitis B (CHB) infection. In this study, we investigated the possible association between TLRs and hepatitis B virus (HBV) infection chronicity in Turkish population. Methods: TLR4 (+896 A→G) (rs4986790), TLR5 (+1846 T→C) (rs5744174) and TLR9 (−1237T→C) (rs5743836) polymorphisms were screened in 131 CHB patient and 168 individuals by polymerase chain reaction (PCR) – restriction fragment length polymorphism (RFLP) technique. Results: Of the screened polymorphisms, TT genotype of the missense variant TLR5 (rs5744174) (NM_003268.5:c.1846T > C (p.Phe616Leu) is significantly more frequent in the control group than CHB patients (P < 0.001), presence of TT genotype of the upstream variant TLR9 (rs5743836) (NM_017442.3:c.−1237T > C) is more frequent in CHB group (P = 0.043). However, no significant association was found for the missense variant TLR4 (rs4986790) NM_138554.4:c.896A > G (p.Asp299Gly) polymorphism and CHB in Turkish population. Conclusions: From all three analyzed SNPs association of TLR5 (rs5744174) with CHB is the most significant. Since TLR5 is associated with interferon-γ production, a high frequency of TT at rs5744174 in controls subjects suggests that it represents a protective genotype against CHB plausibly associated with an increased interferon-γ production.

KW - Chronic hepatitis B

KW - PCR-RFLP

KW - Polymorphism

KW - Seroconversion

KW - Toll-like receptors

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SN - 2210-7401

VL - 42

SP - 139

EP - 144

JO - Clinics and Research in Hepatology and Gastroenterology

JF - Clinics and Research in Hepatology and Gastroenterology

IS - 2

ER -

The association of variations in TLR genes and spontaneous immune control of hepatitis B virus

Abstract

Bibliographical note

Keywords

UN SDGs

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