TY - JOUR
T1 - Surveillance of SARS-CoV-2 RNA in wastewater treatment plants in Türkiye, Istanbul
T2 - a long-term study and statistical analysis
AU - Eryildiz-Yesir, Bahriye
AU - Akdag-Aras, Isilay
AU - Sayi-Yazgan, Ayca
AU - Polat, Ece
AU - Altınbaş, Mahmut
AU - Gul, Bahar Yavuzturk
AU - Koyuncu, Ismail
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer Nature Switzerland AG 2025.
PY - 2025/6
Y1 - 2025/6
N2 - Wastewater-based epidemiology (WBE) is a powerful method that allows community surveillance to identify diseases/pandemic dynamics in a city, especially in metropolitan areas with high overpopulation. This study investigated the detection and quantification of SARS-CoV-2 RNA in wastewater and sewage sludge in two different wastewater treatment plants (WWTPs) in Istanbul, the 5th largest city in the world, during the COVID-19 pandemic. Statistical analysis was performed to examine the relationships between SARS-CoV-2 concentrations and COVID-19 case numbers, seasonal variations, and key WWTP parameters, including total phosphorus (TP), total nitrogen (TN), and chemical oxygen demand (COD), using principal component analysis (PCA). SARS-CoV-2 N genes were analyzed in influent, treated effluent and sludge samples collected between June 2021 and January 2022 by reverse transcription–polymerase chain reaction (RT-qPCR). Viral genes were detected in 23 out of 26 influent wastewater samples (88%) and in 4 out of 26 secondary treated wastewater samples. SARS-CoV-2 RNA concentrations in influent samples ranged from 0 to 11,469 ± 551.2 genomic copies (GC)/mL for all viral genes analyzed (N1: 109.7 ± 46.6 − 11,228.9 ± 543.05 GC/mL and N2: 864.5 ± 82 − 11,469.6 ± 551.2 GC/mL). In secondary wastewater samples, SARS-CoV-2 RNA concentrations ranged from 665.23 ± 48.6 to 2,833.17 GC/mL, while sludge samples contained concentrations ranging from 138.7 ± 20.1 to 2,027.9 ± 97.2 GC/mL. The findings of this work demonstrate the feasibility of RT-qPCR for quantifying SARS-CoV-2 in wastewater and suggest a weak to moderate correlation between SARS-CoV-2 gene concentrations and confirmed COVID-19 and death cases in the community. A correlation was also observed between the number of GC per TP and COD for both the N1 (Pearson correlation coefficient: 0.949 for N1/TP and 0.986 for N1/COD) and N2 genes (Pearson correlation coefficient: 0.960 for N2/TP and 0.993 for N2/COD) in two WWTPs. The study highlights the potential of WBE as a COVID-19 surveillance tool and as an early warning and control system for SARS-CoV-2 infections.
AB - Wastewater-based epidemiology (WBE) is a powerful method that allows community surveillance to identify diseases/pandemic dynamics in a city, especially in metropolitan areas with high overpopulation. This study investigated the detection and quantification of SARS-CoV-2 RNA in wastewater and sewage sludge in two different wastewater treatment plants (WWTPs) in Istanbul, the 5th largest city in the world, during the COVID-19 pandemic. Statistical analysis was performed to examine the relationships between SARS-CoV-2 concentrations and COVID-19 case numbers, seasonal variations, and key WWTP parameters, including total phosphorus (TP), total nitrogen (TN), and chemical oxygen demand (COD), using principal component analysis (PCA). SARS-CoV-2 N genes were analyzed in influent, treated effluent and sludge samples collected between June 2021 and January 2022 by reverse transcription–polymerase chain reaction (RT-qPCR). Viral genes were detected in 23 out of 26 influent wastewater samples (88%) and in 4 out of 26 secondary treated wastewater samples. SARS-CoV-2 RNA concentrations in influent samples ranged from 0 to 11,469 ± 551.2 genomic copies (GC)/mL for all viral genes analyzed (N1: 109.7 ± 46.6 − 11,228.9 ± 543.05 GC/mL and N2: 864.5 ± 82 − 11,469.6 ± 551.2 GC/mL). In secondary wastewater samples, SARS-CoV-2 RNA concentrations ranged from 665.23 ± 48.6 to 2,833.17 GC/mL, while sludge samples contained concentrations ranging from 138.7 ± 20.1 to 2,027.9 ± 97.2 GC/mL. The findings of this work demonstrate the feasibility of RT-qPCR for quantifying SARS-CoV-2 in wastewater and suggest a weak to moderate correlation between SARS-CoV-2 gene concentrations and confirmed COVID-19 and death cases in the community. A correlation was also observed between the number of GC per TP and COD for both the N1 (Pearson correlation coefficient: 0.949 for N1/TP and 0.986 for N1/COD) and N2 genes (Pearson correlation coefficient: 0.960 for N2/TP and 0.993 for N2/COD) in two WWTPs. The study highlights the potential of WBE as a COVID-19 surveillance tool and as an early warning and control system for SARS-CoV-2 infections.
KW - COVID-19
KW - Istanbul
KW - SARS-CoV-2
KW - Wastewater
KW - Wastewater-based epidemiology sludge
UR - https://www.scopus.com/pages/publications/105004741465
U2 - 10.1007/s10661-025-14101-7
DO - 10.1007/s10661-025-14101-7
M3 - Article
AN - SCOPUS:105004741465
SN - 0167-6369
VL - 197
JO - Environmental Monitoring and Assessment
JF - Environmental Monitoring and Assessment
IS - 6
M1 - 647
ER -
