Superresolving masks for incoherent highnumerical-aperture scanning microscopy in three dimensions

Ibrahim Akduman; Ulrich Brand; Jan Grochmalicki; Gerard Hester; Roy Pike; Mario Bertero

doi:10.1364/JOSAA.15.002275

Superresolving masks for incoherent highnumerical-aperture scanning microscopy in three dimensions

Ibrahim Akduman^*, Ulrich Brand, Jan Grochmalicki, Gerard Hester, Roy Pike, Mario Bertero

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

A singular-value-decomposition analysis of the imaging kernel for three-dimensional fluorescent laser scanning microscopy at a high numerical aperture (NA) is presented. The design and superresolving performance of image-plane binary optical masks are then derived, and new computational techniques for calculating these masks are given. Initial experimental results with a microscope equipped with such a mask at NA= 1.3 are presented. The improvement in both contrast and resolution over the confocal and type 1 instruments is demonstrated.

Original language	English
Pages (from-to)	2275-2287
Number of pages	13
Journal	Journal of the Optical Society of America A: Optics and Image Science, and Vision
Volume	15
Issue number	9
DOIs	https://doi.org/10.1364/JOSAA.15.002275
Publication status	Published - Sept 1998
Externally published	Yes

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title = "Superresolving masks for incoherent highnumerical-aperture scanning microscopy in three dimensions",

abstract = "A singular-value-decomposition analysis of the imaging kernel for three-dimensional fluorescent laser scanning microscopy at a high numerical aperture (NA) is presented. The design and superresolving performance of image-plane binary optical masks are then derived, and new computational techniques for calculating these masks are given. Initial experimental results with a microscope equipped with such a mask at NA= 1.3 are presented. The improvement in both contrast and resolution over the confocal and type 1 instruments is demonstrated.",

author = "Ibrahim Akduman and Ulrich Brand and Jan Grochmalicki and Gerard Hester and Roy Pike and Mario Bertero",

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AU - Akduman, Ibrahim

AU - Brand, Ulrich

AU - Grochmalicki, Jan

AU - Hester, Gerard

AU - Pike, Roy

AU - Bertero, Mario

PY - 1998/9

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N2 - A singular-value-decomposition analysis of the imaging kernel for three-dimensional fluorescent laser scanning microscopy at a high numerical aperture (NA) is presented. The design and superresolving performance of image-plane binary optical masks are then derived, and new computational techniques for calculating these masks are given. Initial experimental results with a microscope equipped with such a mask at NA= 1.3 are presented. The improvement in both contrast and resolution over the confocal and type 1 instruments is demonstrated.

AB - A singular-value-decomposition analysis of the imaging kernel for three-dimensional fluorescent laser scanning microscopy at a high numerical aperture (NA) is presented. The design and superresolving performance of image-plane binary optical masks are then derived, and new computational techniques for calculating these masks are given. Initial experimental results with a microscope equipped with such a mask at NA= 1.3 are presented. The improvement in both contrast and resolution over the confocal and type 1 instruments is demonstrated.

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M3 - Article

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SN - 1084-7529

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JF - Journal of the Optical Society of America A: Optics and Image Science, and Vision

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ER -

Superresolving masks for incoherent highnumerical-aperture scanning microscopy in three dimensions

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