Abstract
Objective: To predict the reproductive potential of embryos via Raman spectroscopy evaluation of the spent culture media as well as with a conventional morphologic evaluation. Materials and Methods: Women of reproductive age (n=31) who were treated for unexplained infertility and scheduled for single embryo transfer were invited to participate in this prospective study. After the embryos were removed from the culture, the spent culture media were stored at -80 °C after snap-freezing in liquid nitrogen. Results: Fifteen patients were clinically pregnant, and 16 patients were clinically non-pregnant. Clinical pregnancy was predicted using Raman spectroscopy in 93% (14/15) of clinically pregnant patients, and in 62.5% (10 out of 16) of clinically non-pregnant patients. The sensitivity of the Raman spectroscopic analysis was 93% and the specificity was 62.5%. Conclusion: Metabolomic evaluation of spent embryo culture media is an emerging technique with promising objective results. However, there is clearly room for improvement.
| Translated title of the contribution | In vitro fertilizasyon hastalarının üreme potansiyelini belirlemede embriyo kültür medyasının spektroskopik analizle incelenmesi |
|---|---|
| Original language | English |
| Pages (from-to) | 145-150 |
| Number of pages | 6 |
| Journal | Turkish Journal of Obstetrics and Gynecology |
| Volume | 14 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sept 2017 |
Bibliographical note
Publisher Copyright:© 2017 by Turkish Society of Obstetrics and Gynecology.
Funding
the authors. Financial Disclosure: This research received funding from the Scientific Research Projects Coordination Unit of İstanbul University (grant number: 22501).
| Funders | Funder number |
|---|---|
| İstanbul University | 22501 |
Keywords
- Embryo culture media
- In vitro fertilization
- Morphology
- Raman spectroscopy